相关实验视频

Updated: Aug 9, 2026

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

确定Aptamer sgc8c和PTK7之间的结合点

Jianghuai Chen^1,2, Jiaxuan He², Tao Bing²

¹Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.

Analytical chemistry

|June 18, 2024

概括

研究人员确定了sgc8c胺酶在其标蛋白氨酸激酶7 (PTK7) 上的精确结合部位. 这一发现推动了用于癌症检测和药物输送应用的阿普坦技术的进步.

科学领域:

生物化学生物化学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

胺是核酸分子,具有高度的特异性,用于目标结合.
当前的阿普坦体选方法往往无法准确地绘制结合部位.
了解aptamer-target相互作用对于优化基于aptamer的应用程序至关重要.

研究的目的:

确定 sgc8c 胺酶在其点蛋白氨酸激酶7 (PTK7) 上的确切结合点.
在结合界面上描述 sgc8c 和 PTK7 之间的分子相互作用.

主要方法:

顺序的蛋白质截断来缩小PTK7结合区域.
在体外表达和结晶sgc8c-PTK7结合复合物 (特别是PTK7的Ig3-4).
质谱学,分子动力学模拟和突变分析以确定特定的结合残留物.

主要成果:

sgc8c的结合部位定位在PTK7细胞外域的Ig 3到Ig 4区域.
成功获得了sgc8c-Ig (3-4) 复合物的共晶结构.
确定并验证了PTK7上特定的氨基酸残留物和参与结合的sgc8c上的核酸残留物.

结论:

这项研究精确地绘制了sgc8c合酶和PTK7.7之间的结合接口.
这种详细的表征为 PTK7 相关应用,如癌症诊断和治疗等APTAMER 的合理设计和优化提供了基础.

更多相关视频

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

相关概念视频

Ligand Binding and Linkage

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...