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在非小细胞肺癌患者接受新辅助化疗后,调控性T细胞的空间结构发生变化

Siqi Cai¹, Miaoqing Zhao², Guanqun Yang¹

¹Shandong University Cancer Center, Shandong University, Jinan, Shandong, China; Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.

International immunopharmacology

|June 18, 2024

概括

新辅助化疗增加了非小细胞肺癌 (NSCLC) 的调节性T细胞 (Tregs) 和CD8+T细胞,改善了治疗反应和无复发生存率. 了解Treg空间动态有助于为NSCLC患者提供个性化免疫疗法.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
翻译研究是翻译研究.

背景情况:

调节性T细胞 (Tregs) 在瘤微环境 (TME) 中起着至关重要的作用.
化学疗法对Tregs的调节是改善非小细胞肺癌 (NSCLC) 新辅助化疗免疫疗法的疗效的关键.
了解TME内的Treg相互作用对于NSCLC治疗策略至关重要.

研究的目的:

调查新辅助化疗 (NAC) 对Treg细胞密度,透和与NSCLC中其他免疫和树皮细胞的空间接近的影响.
分析Treg特征与临床结果之间的关联,包括化疗反应和无复发生存率.
探索Treg近距离评分与癌症微血管 (CMV) 和癌症相关纤维细胞 (CAF) 的相关性.

主要方法:

在NAC和仅进行手术的队列中,对II-IIINSCLC患者的瘤样本进行了回顾性分析.
多重光染色 (Foxp3, CD4, CD8, CK, CD31, ɑSMA) 用于评估Treg密度,透和近距离得分 (Dens, InS, TrPS). 通过多重光染色,可以评估Treg密度,透和近距离得分 (Dens, InS, TrPS).
多变量分析以确定Treg指标与治疗反应和无复发生存率的关联.

主要成果:

在NSCLC瘤中,NAC治疗显著增加了Treg密度和透率.
NAC还导致Tregs周围的CD8+T细胞透率增加.
较高的Dens (Tregs),InS (Tregs) 和TrPS (CD8) 与更好的化疗反应和更好的无复发生存率有显著的相关性.
特雷格近距离评分 (TrPS ((CD8),TrPS ((CD4)) 显示与CMV和CAF有负相关性.

结论:

化疗显著调节NSCLC瘤微环境中的Treg景观.
增强的Treg透和接近CD8+T细胞与改善的临床结果相关.
化疗后Tregs的空间分布是NSCLC个性化免疫治疗的潜在生物标志物.

关键词:

肺癌是一种肺癌.多重复合的免疫光.新辅助化学疗法新辅助化学疗法监管T细胞监管T细胞空间建筑的空间架构.

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