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来自Streptomyces roseolus的重组L-Glutaminase的分子表达,净化和结构特征

Zabin K Bagewadi1, Gouri H Illanad1, Ibrahim Ahmed Shaikh2

  • 1Department of Biotechnology, KLE Technological University, Hubballi, Karnataka 580031, India.

International journal of biological macromolecules
|June 18, 2024
PubMed
概括

来自Streptomyces roseolus的重组L-Glutaminase对乳腺癌细胞具有显著的抗癌活性. 这种热稳定酶表现出抗氧化特性和剂量依赖的细胞毒性作用,表明其作为向癌症治疗的潜力.

关键词:
关联性净化净化方法分析性表征分析性表征生物的属性 生物的属性表达方式表达方式表达这是一种L-Glutaminase.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...