相关实验视频

Updated: Jun 23, 2025

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

在鼻癌中YME1L的表达和功能影响

Fuwei Cheng¹, Haiping Huang¹, Shiyao Yin¹

¹Department of Otolaryngology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Cell death & disease

|June 18, 2024

概括

酵母线粒体胺酶1-样 (YME1L) 在鼻癌 (NPC) 中被上调,通过破坏线粒体功能和激活Akt-mTOR通路来驱动瘤生长. 抑制YME1L显示在NPC中具有抗瘤作用.

科学领域:

线粒体生物学线粒体生物学
在瘤学瘤学.
癌症的分子机制癌症的分子机制

背景情况:

线粒体对鼻癌 (NPC) 的进展至关重要.
酵母 mitochondrial amidase 1-like (YME1L),一个AAA ATPase,调节线粒体功能,并与疾病有关.

研究的目的:

研究YME1L在NPC中的表达和功能作用.
阐明YME1L参与NPC瘤发生的分子机制.

主要方法:

分析了NPC组织和细胞中的YME1L表达与正常对照对比.
利用遗传沉默 (shRNA) 和淘汰 (CRISPR-sgRNA) 来评估YME1L在NPC细胞中的功能.
研究了YME1L对线粒体功能,细胞活力,增殖,迁移和亡的影响.
研究了YME1L在Akt-mTOR信号通路中的作用.
在NPC异种移植中使用YME1L-shRNA表达腺相关病毒 (AAV) 进行了体内研究.

主要成果:

在NPC组织和细胞中,YME1L被显著上调.
由于YME1L的枯竭,线粒体功能受损 (脱极化,ROS生成,减少ATP) 并抑制NPC细胞活力,增殖和迁移,同时促进细胞亡.
YME1L过度表达增强了NPC细胞的增殖和迁移.
YME1L积极调节了Akt-mTOR通路;它的耗尽使Akt-S6K酸化失活,该酸化被一个构成性活跃的Akt1突变物所拯救.
在体内,通过AAV抑制YME1L抑制了NPC异种植的生长,与线粒体功能障碍,氧化应激,Akt-mTOR失活和亡相关.

结论:

过度表达的YME1L通过增强的线粒体功能和Akt-mTOR激活促进NPC进展.
向YME1L代表了鼻癌的潜在治疗策略.

更多相关视频

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

Published on: December 20, 2021

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...