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综合转录组和代谢学分析揭示了在Cr压力下Canna indica中的关键功能基因

Sixi Zhu¹, Wei Zhao², Luying Sheng²

¹College of Eco-Environment Engineering, The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, Guizhou Minzu University, Guiyang, 550025, China. zhusixi2011@163.com.

Scientific reports

|June 18, 2024

概括

这项研究揭示了Canna indica随着时间的推移对 (Cr) 污染的反应. 它确定了关键的代谢途径和基因表达变化,突出了植物中的Cr毒性管理策略.

科学领域:

环境科学环境科学
植物生物学植物生物学
生物化学生物化学

背景情况:

(Cr) 污染对植物的基因表达,新陈代谢和生长构成风险.
湿地植物对随着时间的推移对Cr压力的反应的精确分子机制仍然不清楚.

研究的目的:

阐明Canna indica对压力的时间分子反应机制.
在不同的Cr暴露时间下分析基因表达和代谢物概况的变化.

主要方法:

使用非向代谢学 (LC-MS) 和转录学.
卡纳印加被暴露在100mg/kg的Cr污染土壤中,持续时间为0,7,14,21天.
分析包括酶活性,代谢物含量和基因表达特征.

主要成果:

抗氧化应激增加了抗氧化酶活性 (SOD,APX,POD),谷氨 (GSH),氨 (MDA) 和活性氧物种 (ROS),同时抑制了光合作用颜料.
代谢学发现了12种受影响的途径和38种差异表达的代谢物 (氨基酸,烯,黄).
转录学揭示了16,247个差异表达基因 (DEGs);早期的压力涉及银糖,粉和糖代谢,中期受影响的植物激素和MAPK信号传递,以及晚期激活的谷氨代谢和烯胺生物合成.

结论:

甘印加采用不同的分子策略,以应对不同暴露时间的毒性.
早期反应侧重于基本代谢,转向信号通路,最后转向解毒机制,如谷氨代谢和烯胺生物合成.

关键词:

卡纳可纳指标 (Canna indica) 是一个指标.(Chromium) 是一种含的物质.代谢组中的代谢.生理学生理学生理学转录组转录组就是一个转录组.

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