背侧侧生殖细胞核和胸膜网状细胞核之间的互联连接的发展
Peter W Campbell1,2, Gubbi Govindaiah1, William Guido3
1Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, 511 S. Floyd St., Louisville, KY, 40292, USA.
Neural development
|June 18, 2024
概括
在小鼠中,研究了乳头网状核 (TRN) 与背侧生殖核 (dLGN) 之间的连接的发展. 研究人员发现,这些关键电路在出生后逐渐成熟,影响感官处理和神经发育障碍.
科学领域:
- 神经科学是一个神经科学.
- 发育神经科学的发展神经科学.
- 系统神经科学 系统神经科学
背景情况:
- 胸膜网状核 (TRN) 是连接胸膜和新皮质的关键枢纽,调节感官处理和胸膜皮质节律.
- 在TRN电路中的功能障碍与神经发育障碍,如,自闭症和注意力缺陷有关.
- 发展时间表和TRN与第一阶梯核之间的连接的功能成熟,比如背侧生殖核 (dLGN),仍然不太清楚.
研究的目的:
- 在小鼠模型中研究TRN和DLGN之间的电路的发育轨迹,内化模式和功能成熟.
- 阐明视网膜输入和内在发育程序在塑造这些thalamic内连接中的作用.
主要方法:
- 利用基因改造的小鼠线路可视化和准前 (甲状腺皮层到TRN) 和反 (TRN到甲状腺) 电路.
- 采用逆行追踪来分析甲状腺皮层投影及其组织.
- 在各种出生后年龄段使用急性乳头切片制剂和光遗传刺激评估功能性突触反应.
主要成果:
- 通过TRN的thalamocortical投射在出生时就存在,而前进的TRN内化则在出生后的第二周出现,并在第三周成熟.
- 从头皮层输入到TRN中的功能激发反应从第1周到第4周加强并变得更加普遍.
- 从TRN到dLGN的反抑制预测出现在出生后的第二天,在前四周加强并开始抑制甲状腺皮层尖端.
- 视网膜输入的缺失加速了DLGN的TRN内化,但对DLGN到TRN投射的影响很小.
结论:
- 这项研究揭示了在出生后早期发育过程中体内电路出现的精确时间和机制.
- 这些发现为了解皮层网络动态和与TRN功能障碍相关的神经发育障碍提供了基础知识.
- 视网膜输入对视觉 thalamus 中的前和反电路发展有不同的影响.
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