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Updated: Jun 24, 2026

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一个3D打印的集成手持式生物传感器用于检测Vibrio parahaemolyticus

Yuancong Xu^1,2, Qian Zhang^2,3, Yunyi Li³

¹College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China.

Foods (Basel, Switzerland)

|June 19, 2024

概括

一个新的3D打印生物传感器可以高灵敏地检测出Vibrio parahaemolyticus. 这种集成的手持生物传感器 (IHB) 使用RPA-CRISPR/Cas12a和侧流条来快速选海鲜中的病原体.

科学领域:

食品安全食品安全
生物传感器技术技术
分子诊断学分子诊断学

背景情况:

维布里奥帕拉海莫利蒂库斯 (Vibrio parahaemolyticus) 是一种由海鲜传播的重要病原体,会导致人类的胃肠道疾病.
生物传感器可以精确检测食源性病原体,但需要低成本的便携式设备.
目前的检测方法可能耗时,需要专门的设备.

研究的目的:

开发一个低成本的,集成的手持式生物传感器 (IHB),用于超敏感检测Vibrio parahaemolyticus.
在一个便携式设备中将复合酶聚合酶放大 (RPA),CRISPR/Cas12a和侧流条 (LFS) 结合起来.
为了能够在食品样本中快速准确地选Vibrio parahaemolyticus.

主要方法:

开发了一个3D打印的IHB,集成RPA,CRISPR/Cas12a和LFS.
使用RPA预放大了Vibrio parahaemolyticus的Thl基因.
使用CRISPR/Cas12a跨裂变活动通过LFS检测放大DNA.

主要成果:

IHB在检测Vibrio parahaemolyticus方面表现出高的选择性和出色的灵敏度.
达到4.9 CFU/mL的超敏感检测极限.
该系统显示了放大RPA产品和LFS信号强度之间的负相关性.

结论:

开发的IHB是对样本中Vibrio parahaemolyticus的超敏感和快速查的一个有前途的工具.
这项技术有可能在监测各种病原体风险的海鲜和海洋环境安全方面得到更广泛的应用.
IHB的低成本,便携性使其在食品安全应用中得到广泛使用.

关键词:

3D打印的生物传感器这就是RPA-CRISPR/Cas12a.这种病毒是Vibrio parahaemolyticus.侧向流动条带是一个侧向流动条.快速检测快速检测迅速检测

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