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由除草剂葡萄酸诱导的细胞和基因组不稳定性:一个体外和体外的方法

Alfredo Santovito¹, Mattia Lambertini², Dáša Schleicherová¹

¹Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina 13, 10123 Torino, Italy.

|June 19, 2024

概括

草药酸氨酸 (GLA) 会导致DNA损伤,并减少人类淋巴细胞和牛血细胞中的细胞增殖. 它还对牛生长和蛋生产产生了负面影响,这表明了对环境有毒性的担忧.

科学领域:

环境毒理学环境毒理学
遗传学遗传学是一个
生态毒理学生态毒理学

背景情况:

酸氨酸 (GLA) 是一种有机酸除草剂,广泛释放到环境中.
对其潜在的基因毒性和细胞效应存在担忧,但研究是有限的.
了解GLA对非标生物和人类细胞的影响至关重要.

研究的目的:

评估GLA对人类淋巴细胞和Lymnaea stagnalis*血细胞的基因毒性.
评估GLA对细胞增殖的细胞毒性影响.
调查GLA对Lymnaea stagnalis的生殖健康和生长的影响.

主要方法:

在体外和体内微核测定是在培养的人类淋巴细胞和L. stagnalis*血细胞上进行的.
测试了六种不同的GLA度,范围从0.010到0.500微克/毫升.
还测量了L. stagnalis*的细胞增殖,卵子生产和身体生长.

主要成果:

在人体淋巴细胞中,GLA显著增加了微核的频率,其度≥0.100μg/mL,在L. stagnalis*血细胞中,其度≥0.200μg/mL.
在大多数测试的GLA度中观察到细胞增殖的显著减少.
在暴露于GLA的*L. stagnalis*中,观察到卵产量和身体生长的减少.

结论:

在人类淋巴细胞和L. stagnalis*血细胞中,GLA会诱导基因组和细胞损伤.
这种除草剂表现出细胞毒性作用,损害细胞增殖.
GLA对*L. stagnalis*的生殖健康和生长产生负面影响,突出了其生态毒理学风险.

关键词:

生态毒理学生态毒理学基因毒理学基因毒理学微核是微核中的一个.核武器的朋友们有机酸盐是一种有机酸

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