相关实验视频
Updated: Jun 23, 2025
10:36
Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
13.9K
线粒体蛋白编码基因表达 在 (Sphenomorphus incognitus) (Squamata:Scincidae) 响应不同的温度压力
Lemei Zhan1, Jingyi He1, Siqi Meng1
1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
Animals : an open access journal from MDPI
|June 19, 2024
概括
全球变暖影响爬行动物生理学. 这项研究表明,棕色森林皮改变了线粒体基因表达,以应对极端的寒冷和热,突出了线粒体的功能.
科学领域:
- 生态学和进化生物学
- 分子生物学分子生物学
- 环境生理学环境生理学
背景情况:
- 全球变暖增加了极端天气事件,威胁到等外热生物.
- 爬行动物生理和行为对温度波动高度敏感.
- 线粒体对于能量代谢和保护细胞免受温度压力至关重要.
研究的目的:
- 研究极度寒冷 (4°C,8°C) 和高温 (34°C,38°C) 对棕色森林皮 (Sphenomorphus incognitus) 中线粒体基因表达的影响.
- 了解线粒体功能在爬行动物的热可塑性和适应策略中的作用.
主要方法:
- 使用定量实时PCR (RT-qPCR) 分析了13个线粒体蛋白质编码基因 (PCG) 的表达模式.
- 在24小时暴露于对照 (25°C) 和极端温度 (4°C,8°C,34°C,38°C) 后,基因表达在Sphenomorphus incognitus中被测量.
主要成果:
- 低温 (4°C) 导致了几种线粒体PCG (例如COI,ND4L) 的显著下调和COIII的上调.
- 暴露于8°C还导致特定线粒体PCG (例如ND1,ND3,ND4) 的下调.
- 高温 (34°C和38°C) 诱导了各种线粒体PCG (例如COI,COII,ND6) 的显著上调,38°C显示了大多数基因的广泛增加.
结论:
- 线粒体基因表达模式在S. 在冷和热应激下,incognitus 显著变化.
- 这些变异表明适应性策略涉及线粒体功能快速补偿性调整,以保持热恒温.
- 线粒体功能在爬行动物的热可塑性中起着至关重要的作用,使其能够适应不断变化的环境温度.
更多相关视频
08:11Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris
Published on: January 3, 2025
424
12:38Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
Published on: December 18, 2013
6.1K
相关概念视频
02:27Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
02:45Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K