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来自内皮细胞的微小水的表面甘氨酸,用植物莱克进行探测

Ekaterina V Slivka¹, Nadezhda V Shilova^1,2, Ekaterina A Obraztsova¹

¹Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10 Miklukho-Maklaya Str., Moscow 117997, Russia.

International journal of molecular sciences

|June 19, 2024

概括

由内皮细胞衍生的微 (MVs) 显示了特定的N-甘氨酸配置文件,主要由橄果酸胺和α2-6-化形式. 这表明非随机的蛋白质集成到MVs基于糖化.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
格莱科米克斯 (Glycomics) 是一个有趣的药物.

背景情况:

微 (MVs) 涉及细胞间通信,其甘氨酸可能调解向特异性.
与外体相比,对MV的糖体研究很少.
了解MV糖基化对于破译它们的生物作用至关重要.

研究的目的:

为了描述内皮细胞衍生MVs的N-甘氨酸概况.
为了研究融入MVs中的蛋白质的糖化模式.
探索观察到的甘氨酸水平背后的潜在机制.

主要方法:

用了21种不同的植物学菌素来对MVs进行糖分析.
分析了N-甘氨酸,并研究了糖脂类的潜在作用.
与潜在的细胞外影响相比较的MV糖分谱.

主要成果:

内皮 MVs 呈现出橄果酸胺和α2-6-化N-甘氨酸的优势.
观察到低水平的α2-3-sialylated glycans,不能归因于细胞外糖化酶.
在MVs中发现了某些含有曼诺斯的甘氨酸水平的降低.
综合蛋白或蛋白质甘氨酸的空间掩盖被认为不太可能解释低水平的甘氨酸.

结论:

内皮膜MVs具有明显的N-甘氨酸签名.
观察到的甘氨酸概况表明,蛋白质被选择性地纳入MVs.
蛋白质集成到MVs可能受到特定的糖化模式的影响,而不是随机.

关键词:

内皮细胞是内皮细胞.细胞外囊泡中的细胞外囊泡.糖甘油的使用方法葡萄糖脂类的葡萄糖脂类.微小的微小的微小的微小的微小

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