三种新型Serratia和Enterobacter特定病毒性菌体的生物特征和基因组分析
Dziyana Shymialevich1, Stanisław Błażejak2, Paulina Średnicka3
1Culture Collection of Industrial Microorganisms-Microbiological Resources Center, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland.
International journal of molecular sciences
|June 19, 2024
概括
针对塞拉提亚和肠杆菌物种的三种新型细菌菌被描述为食品安全应用. 这些菌体表现出广泛的温度和pH稳定性,缺乏有害基因,显示出在食品中的生物控制潜力.
科学领域:
- 食品微生物学与食品安全
- 菌体生物学和基因组学
- 降低抗微生物药物耐药性的缓解
背景情况:
- 食品原料中微生物污染的增加需要新的安全策略.
- 越来越多的抗药性细菌对公众健康构成重大威胁.
- 菌体 (菌体) 为控制食品中的细菌病原体提供了一个有前途的生物解决方案.
研究的目的:
- 在生物学和基因组学上对三种新分离的毒性细菌菌体进行表征.
- 评估这些菌体在食品工业中的生物控制应用中的潜力.
- 通过分析其基因组的毒性和毒素基因来评估分离的菌体的安全性.
主要方法:
- 从市政污水中分离和鉴定特定于Serratia和Enterobacter物种的细菌菌体.
- 在广泛的温度范围 (-20°C至60°C) 和pH值 (3至12) 中评估菌体活动.
- 传输电子显微镜 (TEM) 用于形态分析和全基因组测序 (WGS) 用于基因组表征.
主要成果:
- 三种毒性细菌菌体 (Serratia菌体KKP_3708,Serratia菌体KKP_3709和Enterobacter菌体KKP_3711) 被分离并进行了表征.
- 菌体在广泛的温度和pH值范围内表现出强大的活性,表明它们适合于各种食物环境.
- 基因组分析证实菌体属于Caudoviricetes类,具有线性双链DNA基因组,没有检测到毒性,毒素或抗生素耐药性基因.
结论:
- 鉴定出来的菌体对 Serratia 和 Enterobacter 的菌株有效.
- 它们在各种环境条件下的稳定性和没有有害的遗传元素支持它们在食品生物控制中的使用.
- 缺乏 lysogenic 标记物进一步提高了它们在食品工业潜在部署的安全性.
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