基尿素对形态,增殖和蛋白质表达在Taenia crassiceps WFU菌株上的影响
Diana G Rios-Valencia1, Karel Estrada2, Arturo Calderón-Gallegos3
1Department of Microbiology and Parasitology, School of Medicine, Universidad Nacional Autónoma de México, Coyoacan, Mexico City 04510, Mexico.
International journal of molecular sciences
|June 19, 2024
概括
氧尿素 (HU) 治疗会消耗条虫中的生殖细胞,影响再生. 治疗后观察到这些细胞的部分恢复,蛋白质组分析显示了关键的分子变化.
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 平虫,特别是像T.crassiceps这样的虫,具有显著的再生能力.
- 这种再生依赖于全能发芽细胞,其枯竭会影响寄生虫再生的能力.
- 已知基尿素 (HU) 会耗尽生殖细胞,从而影响细胞的再生.
研究的目的:
- 为了研究在*T.crassiceps* cysticerci中以基尿素 (HU) 进行体外治疗后的发芽细胞的减少和随后的恢复.
- 评估HU对寄生虫生存能力,形态,移动性和细胞增殖的影响.
- 通过蛋白质和基因本体学分析,识别受HU治疗影响的分子通路.
主要方法:
- 在体外培养T.crassiceps* cysticerci,其HU度各不相同 (25mM和40mM),持续6天.
- 评估治疗后和康复期间 (3天和6天) 的囊活力,形态和运动能力.
- 使用EDU结合和蛋白质组分析加上基因本体学丰富的增殖细胞的量化.
主要成果:
- 较高的HU度 (40mM) 诱导了发育的囊细胞的显著形态变化.
- 治疗6天后,在两种HU度下观察到囊细胞的运动减少.
- 治疗后3天和6天,与25mM HU进行治疗后,增殖细胞的部分恢复被观察到.
- 蛋白质组分析揭示了与DNA结合,DNA损伤,糖解,细胞骨和RNA结合相关的蛋白质的变化.
结论:
- 基尿素治疗有效地减少了T.crassiceps的发芽细胞,损害了寄生虫的移动性和形态.
- 在HU度较低后,繁殖能力的部分恢复发生,这表明弹性.
- 观察到的蛋白质变化突出了受HU影响的关键细胞过程,为再生机制和寄生虫对破坏DNA的反应提供了洞察力.
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