甲状腺菌 (Streptococcus gordonii) 超菌口腔感染引起的牙周炎和强大的miRNA表达动力学
Chairmandurai Aravindraja1, Syam Jeepipalli1, William D Duncan2
1Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USA.
International journal of molecular sciences
|June 19, 2024
概括
在小鼠中感染Streptococcus gordonii会导致显著的膜骨再吸收和改变microRNA (miRNA) 表达. 机器学习确定了与感染严重程度相关的关键miRNA,为牙周病提供了潜在的诊断标记.
科学领域:
- 口腔微生物学 口腔微生物学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 甲状腺菌是口腔健康中的一个开始性细菌.
- 微RNAs (miRNAs) 与牙周病 (PD) 病原发生有关.
- 肺炎是一种由口腔微生物引起的炎症性疾病.
研究的目的:
- 在感染Streptococcus gordonii的小鼠中研究全球miRNA表达动力学.
- 在应对S. gordonii感染时识别差异表达的miRNA.
- 使用机器学习将miRNA表达与膜骨再吸收 (ABR) 相关联.
主要方法:
- C57BL/6J小鼠在8周和16周被S. gordonii感染.
- 评估了牙殖民和气囊骨再吸收.
- 进行了小鼠下的miRNA分析.
- 五个机器学习算法被用于分析miRNA表达数据.
主要成果:
- S. gordonii感染导致确认了牙殖民和明显的下/上ABR (p < 0.0001).
- 在8周,有191个miRNA被上调和22个被下调;在16周,有10个被上调和32个被下调.
- 特定的miRNAs (例如,miR-210,miR-423-5p,miR-2135,miR-145) 显示出一致的表达变化.
- 机器学习确定了miR-22,miR-30c,miR-720和miR-339-5p在与S. gordonii感染的miRNA副本相关联方面具有影响力.
结论:
- 在小鼠中,S. gordonii感染会诱导显著的膜骨再吸收.
- 随着时间的推移,不同的miRNA表达特征与S. gordonii感染有关.
- 已识别的miRNA具有作为S. gordonii相关牙周病的生物标志物的潜力.
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