在葡萄藤中转录因子WRKY的全基因组识别,特征和演变 (Vitis vinifera):新的观点和更新
Ekaterina Vodiasova1,2, Anastasiya Sinchenko1, Pavel Khvatkov1
1Federal State Funded Institution of Science "The Labor Red Banner Order Nikita Botanical Gardens-National Scientific Center of the RAS", Nikita, 298648 Yalta, Russia.
International journal of molecular sciences
|June 19, 2024
概括
这项研究确定并编号了62个葡萄藤WRKY基因,揭示了新奇的化学基因和氨基酸变异性. 为WRKY转录因子提出了一个新的进化模型.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 进化生物学是进化的生物学.
背景情况:
- WRKY转录因子调节植物发育和应激反应.
- 葡萄树中WRKY基因的进化和特定作用尚不清楚.
- 在葡萄藤中不一致的WRKY基因命名导致了混乱.
研究的目的:
- 为了识别和系统地编号所有WRKY基因在Vitis vinifera.
- 分析 WRKY 基因结构,氨基酸变异性和家族遗传关系.
- 为了研究葡萄藤中WRKY基因的进化历史.
主要方法:
- 在六种品种中对62种Vitis vinifera WRKY基因进行全基因组识别.
- 基于染色体位置的标准化编号.
- 对基因结构,氨基酸序列和家族遗传学类的分析.
- 识别仿真WRKY基因及其域.
主要成果:
- 一个完整的 62 个葡萄酒 WRKY 基因与修订编号的目录.
- 在一些 WRKY 中观察到跨品种的显著氨基酸变异 (>5%).
- 发现了具有独特域的仿真WRKY基因 (例如VvWRKY17,VvWRKY61).
- 遗传学分析揭示了与WRKY群体 (I-III) 相对应的五个类 (A-E).
结论:
- 这项研究为葡萄藤WRKY基因提供了一个统一的命名体系.
- 对WRKY基因结构,变异性和维蒂斯葡萄酒的进化进行了新的洞察.
- 一个为WRKY基因提出的进化模型,具有复杂性和简化的动态阶段.
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