通过聚乙烯醇增强黄素的溶解性,稳定性,细胞吸收和生物活性
Smee Kang1, Minkyoung Kim1, Hyelin Kim1
1Department of Food Science and Technology, College of Science and Convergence Technology, Seoul Women's University, 621 Hwarang-ro, Nowon-gu, Seoul 01797, Republic of Korea.
International journal of molecular sciences
|June 19, 2024
概括
聚乙醇 (PVA) 显著提高了黄素在水系中的可溶性,稳定性和细胞吸收. 这种改善增强了黄素的抗氧化和细胞毒性活动,增加了其生物可用性和药理疗效.
科学领域:
- 药理学和制药学 药理学和制药学
- 自然产品化学 自然产品化学
- 生物化学 生物化学
背景情况:
- 黄是一种来自黄 (Curcuma longa) 的强效多,表现出多样化的生物活性.
- 较差的水溶性限制了黄素在生物系统中的生物利用和实验分析.
- 调查配方策略以克服黄素的可溶性限制对于其治疗应用至关重要.
研究的目的:
- 评估聚乙醇 (PVA) 对黄素溶解度,稳定性,细胞吸收和生物活性的影响.
- 确定PVA是否可以在水性环境中增强黄素的药理疗效.
- 评估PVA作为改善黄素输送和性能的潜在载体.
主要方法:
- 在不同度PVA的存在下评估黄素的溶解性和光谱特性.
- 用PVA评估曲素水溶液的悬浮和光稳定性.
- 量化了抗氧化活性 (DPPH,ABTS,AAPH基,氧化清理) 和对HCT 116和INT 407细胞的细胞毒性作用.
- 在INT 407细胞中使用PVA测量了黄素的细胞吸收.
主要成果:
- PVA显著增加了黄素的溶解度,并在水溶液中改变了其光谱特征.
- PVA增强了黄素的悬浮稳定性和光稳定性,即使在低度下也是如此.
- 库尔库的抗氧化和氧化清除活性被PVA显著增加.
- PVA降低了黄素对结肠癌 (HCT 116) 和肠道 (INT 407) 细胞的细胞毒性IC50值.
- PVA以度依赖的方式改善了黄素的细胞吸收,特别是在较低度的黄素时.
结论:
- 聚乙烯醇有效地提高了黄素在水系统中的溶解性和稳定性.
- 这些由PVA调解的物理化学改进导致黄素增强的抗氧化,细胞毒性和细胞吸收特性.
- 作为一种配方载体,PVA有望提高黄素的生物可用性和药理疗效.
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