含有甲基和多德的益生菌饮食在雄性大鼠肠道微生物生态系统中产生了动态和可重现的变化
Robert S Thompson1,2, Samuel J Bowers3, Fernando Vargas4
1Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, USA.
Nutrients
|June 19, 2024
概括
益生菌,如银河和多 (PDX) 持续改变肠道细菌和代谢物,促进更健康的肠道微生物群. 这些变化可以在不同研究地点重现.
科学领域:
- 微生物学 微生物学
- 营养科学 营养科学
- 代谢学 代谢学 代谢学
背景情况:
- 众所周知,益生菌有益于宿主健康和应激弹性.
- 然而,由于益生菌导致的肠道微生物生态和代谢物的动态变化尚未得到充分记录.
- 益生菌对特定细菌和代谢物的作用的可重现性仍然是一个挑战.
研究的目的:
- 为了研究益生菌饮食 (银醇糖 (GOS) 和多糖 (PDX)) 对肠道微生物群和微生物修饰的胆酸随着时间的推移而产生的一致影响.
- 评估这些效应在两个独立研究站点的可重现性.
- 探索微生物代谢途径和胆酸修饰之间的关系.
主要方法:
- 雄性Sprague Dawley大鼠被食为对照或富含益生菌的饮食.
- 使用16S rRNA基因测序分析了肠道微生物组.
- 代谢体,特别是微生物修饰的胆酸,使用非向的LC-MS/MS.进行了分析.
- 使用PICRUSt2.2预测了推断的微生物功能.
主要成果:
- 益生菌饮食显著改变了肠道微生物的β多样性和随着时间的推移而变化的细菌属的相对丰度.
- 微生物修饰的胆汁酸概况一直受到益生菌消费的影响.
- PICRUSt2确定了四个由益生菌影响的关键代谢途径,其中脱氧胆酸成为潜在的网络枢纽.
- 所有观察到的效应在两个研究地点之间都是一致的.
结论:
- 饮食中的益生菌 (GOS/PDX) 强大调节肠道微生物生态系统及其代谢输出.
- 这些发现支持益生菌在建立促进健康的肠道微生物群中的作用.
- 跨站点的一致效应提高了益生菌干预措施对宿主健康和应激恢复能力的可靠性.
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