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在Salvia atropatanaBunge的中改善生物活性多积累

Izabela Grzegorczyk-Karolak¹, Wiktoria Ejsmont¹, Anna Karolina Kiss²

¹Department of Biology and Pharmaceutical Botany, Medical University of Lodz, 90-151 Lodz, Poland.

Molecules (Basel, Switzerland)

|June 19, 2024

概括

萨尔维亚亚亚特罗帕塔纳的培养物被优化为马林酸的生产. 白色和蓝色发光二极管 (LED) 显著提高了生物质和多含量,显示出强大的抗氧化能力.

科学领域:

植物生物技术植物生物技术
药用植物研究药用植物研究
二次代谢物生产二次代谢物生产

背景情况:

(Salvia atropatana) 是一种具有潜在治疗功能的伊朗药用植物.
培养提供了一个可扩展的系统,用于生产植物衍生化合物.
优化培养条件对于最大限度地提高二次代谢物产量至关重要.

研究的目的:

建立和优化Salvia atropatana的菌培养物,以生产罗斯马林酸.
研究发光二极管 (LED) 对生物质积累和多含量的影响.
为了评估发达的系统的抗氧化潜力.

主要方法:

使用Murashige和Skoog (MS) 介质与NAA和CPPU进行感应.
通过超高性能液态染色学-质谱学 (UPLC-MS) 和高性能液态染色学 (HPLC) 进行化合物分析.
培养物的暴露在不同的LED光处理和光灯中进行比较.
使用ABTS,DPPH和FRAP试验进行体外抗氧化活性评估.

主要成果:

CPPU诱导的菌培养显示出有前途的增长,菌体衍生的线条呈现出最高的增长指数和多含量.
确定了12种酸衍生物,其中罗斯马林酸占主导地位.
与光灯相比,白色和蓝色LED显著增强了迷酸和总含量.
优化的体表现出显著的体外抗氧化活性.

结论:

萨尔维亚 (Salvia atropatana) 的系统是一个可行的平台,可以增强生酸的生产.
优化的LED照明条件,特别是白色和蓝色光,可以提高生物质和二次代谢物产量.
发达的系统提供了高质量的植物性产品的来源,具有抗氧化特性.

关键词:

照明灯LED是指LED灯.无生物压力是无生物压力.卡洛斯文化卡洛斯文化这是光谱的光谱.选择线路的选择.芬醇酸是什么芬醇酸是什么酸是什么罗斯马林酸是一种酸.

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