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Updated: Jun 23, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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一种基于强度方程传输的增强型多模相位成像方法

Hong Cheng1, HongYi Zhang1, Wei Lu2

  • 1Key Laboratory of Intelligent Computing & Signal Processing, Anhui University, Hefei, Anhui, China.

Journal of biophotonics
|June 19, 2024
PubMed
概括

这项研究引入了一种先进的无标签细胞成像技术,使用强度方程 (TIE) 的运输来增强对比度. 该方法改善了Zernike相对比 (ZPC) 成像,提供了对未染色的生物样本的更清晰可视化.

关键词:
这是一个计算ZPC.同位基差差异干扰对比度对比度.显微镜成像成像技术多模式相位成像多模式相位成像运输强度方程 (TIE) 的运输

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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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