相关实验视频

Updated: Jun 23, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

纤维细胞生长因子21是一种参与MASLD进展的肝激素

Rocío Gallego-Durán^1,2, Javier Ampuero^1,2,3, Douglas Maya-Miles^1,2

¹SeLiver Group, Instituto de Biomedicina de Sevilla, IBiS/Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain.

United European gastroenterology journal

|June 19, 2024

概括

纤维细胞生长因子21 (FGF21) 在与代谢功能障碍相关的脂肪性肝病 (MASLD) 中被上调. 在MASLD患者中,FGF21水平和rs838133变异与脂肪肝炎和纤维化风险增加有关.

科学领域:

肝病学肝病学是一种肝病学.
代谢疾病代谢疾病
分子生物学分子生物学

背景情况:

与代谢功能障碍相关的脂肪性肝病 (MASLD) 是一个日益严重的健康问题.
纤维细胞生长因子21 (FGF21) 在新陈代谢调节中起作用.
了解FGF21在MASLD中的作用对于开发向疗法至关重要.

研究的目的:

在多个生物尺度上调查FGF21在MASLD中的作用.
在人类MASLD样本和动物模型中分析FGF21基因和蛋白质表达.
检查FGF21遗传变异与MASLD严重程度的关联.

主要方法:

在人类MASLD肝脏样本上进行基因表达分析 (qPCR,RNA-Seq).
在患者血清中测量循环FGF21水平.
在MASLD队列中FGF21 rs838133变异的基因型定型.
在体外研究中,使用暴露于自由脂肪酸 (FFAs) 的肝细胞细胞系.
在体内研究使用小鼠模型 (CDA-HFD) 的脂肪肝炎.

主要成果:

与对照人群相比,MASLD肝脏中FGF21mRNA的显著上调.
在患有脂肪肝炎的患者中增加循环FGF21水平,而不是平淡的脂肪炎.
暴露于FFAs的肝细胞和接受CDA-HFD饮食的小鼠中的FGF21过度表达.
FGF21 rs838133 A基因基因与脂肪肝炎和纤维化风险的增加有关.

结论:

肝脏和循环FGF21表达在MASLD中升高,特别是在脂肪肝炎中.
在MASLD的发病过程中,FGF21起着重要作用.
FGF21 rs838133变种是MASLD严重程度和进展的潜在生物标志物.

关键词:

FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21FGF21 马什 (MASH) 是一个非常重要的产品.马斯尔德马斯尔德是什么意思这是NAFLD.纳什·纳什 (Nash Nash) 是一个名为纳什的城市.纤维细胞生长因子 21 21 基因表达的基因表达方式肝胺 (hepatokine) 是一种肝素.代谢综合征代谢综合征型肝炎 (steatohepatitis) 是一种严重的肝炎.

更多相关视频

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

Published on: February 3, 2023

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Published on: May 13, 2016

相关概念视频

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...