相关实验视频

Updated: Jun 23, 2025

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

血清蛋白质组分析揭示了抑郁症的新型血清生物标志物

Aihong Guo^1,2, Bingju Wang^1,2,3, Jiangbo Ding^1,2

¹Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Frontiers in psychiatry

|June 19, 2024

概括

这项研究确定了四种血清蛋白 - - 蛋白β链,Inter-alpha-trypsin抑制剂重链H4,补充成分3和补充C4A - - 作为抑郁症的潜在生物标志物,有助于早期诊断和治疗.

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
精神病学是一个精神病学.

背景情况:

抑郁症的诊断缺乏客观的生物标志物,依赖于临床评估.
早期发现抑郁症对于改善患者的治疗结果至关重要.
这项研究研究了新型抑郁症生物标志物的血清蛋白质组概况.

研究的目的:

识别和验证与抑郁症相关的血清蛋白生物标志物.
探索已识别的蛋白质在区分抑郁症患者与健康对照中的诊断潜力.
建立一个开发抑郁症客观诊断工具的基础.

主要方法:

使用MB-WCX和MALDI-TOF-MS对抑郁症患者和健康对照的血清样本进行蛋白质组分析.
使用ClinProTools进行差异性峰值查和通过LC-MS/MS识别蛋白质.
在单独的队列和后勤回归分析中对已识别的蛋白质 (TUBB,ITIH4,C3,C4A) 进行ELISA验证.

主要成果:

在血清中确定了五个潜在的与抑郁症相关的峰值.
证实了四种蛋白质Tubulin β 链 (TUBB),Inter-alpha-trypsin 抑制剂重链H4 (ITIH4),补充成分3 (C3) 和补充C4A (C4A).
血清中TUBB,ITIH4,C3和C4A水平升高是抑郁症的重要独立风险因素.

结论:

血清蛋白质学发现TUBB,ITIH4,C3和C4A是潜在的抑郁症生物标志物.
这些蛋白质可以区分抑郁症患者和健康个体.
这些发现可能会促进早期抑郁症诊断和个性化治疗策略.

关键词:

生物标志物生物标志物抑郁抑郁症抑郁症抑郁症抑郁症早期诊断早期诊断早期诊断质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量血液中的血清是血清.

更多相关视频

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

相关概念视频

Depression: Overview

Depression: Overview

Depression is a prevalent mental illness marked by persistent sadness and lack of interest in previously enjoyable activities. It can take several forms, including major depression, persistent depressive disorder, and bipolar I and II disorders. Symptoms range from emotional changes like chronic worry to physical changes like sleep disturbances and suicidal thoughts. From a neurobiological perspective, depression is believed to be triggered by abnormalities in the brain's prefrontal cortex,...

G-protein Coupled Receptors

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...