相关实验视频

Updated: Jun 23, 2025

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

增强的IL-12转基因表达改善了性病毒免疫疗法

Yeaseul Kim¹, Uksha Saini¹, Doyeon Kim¹

¹Center for Childhood Cancer Research, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, United States.

Frontiers in immunology

|June 19, 2024

概括

瘤性简单疹病毒 (oHSV) 对恶性外围神经膜瘤 (MPNSTs) 的治疗不仅取决于病毒复制. 介素-12 (IL-12) 蛋白质产生的差异在体内显著影响免疫反应和瘤抑制.

科学领域:

在瘤学瘤学.
病毒学病毒学
免疫治疗是一种免疫疗法.

背景情况:

恶性外周神经膜瘤 (MPNSTs) 具有攻击性,预后不佳,特别是在神经纤维化1患者中.
瘤性病毒,特别是瘤性简单疹病毒 (oHSV),正在作为MPNST的潜在免疫治疗策略进行研究.

研究的目的:

在容许性和耐药性MPNST模型中比较无武器和与IL-12 (IL-12) 武装的oHSV的疗效.
评估病毒复制,功能活性和宿主免疫反应对体内和体外oHSV治疗.

主要方法:

两种弱化IL-12武装的oHSV与γ134.5基因删除进行了比较:M002和C002 (表达HCMV IRS1基因以逃避转化停止).
病毒复制,转基因表达和免疫细胞透被评估在病毒允许性 (B109) 和抗性 (67C-4) 的小鼠MPNST模型中.

主要成果:

试验室病毒复制没有预测体内瘤学活性;体内抗病毒复制的瘤也限制了转基因表达.
尽管转录活性相似,但oHSV在体内表现出差异性的IL-12蛋白质产生,影响治疗结果.
C002治疗导致持续的IL-12产生,增强免疫细胞活性 (树突细胞,巨细胞) 和多功能Th1细胞反应,导致更大的瘤生长抑制.

结论:

转基因蛋白质的产生,而不仅仅是病毒复制,对于MPNSTs的oHSV治疗疗效至关重要.
IL-12蛋白水平直接影响oHSV治疗的MPNST的抗瘤免疫反应和治疗结果.

关键词:

CD4+ T 细胞是 CD4+ T 细胞.在IL-12中,IL-12是最重要的.干扰素 - 玛的干扰素.在MHC-II上调调节.树突细胞是一种树突细胞.恶性外围神经膜瘤 (MPNST) 是一种恶性外围神经瘤.瘤性简单性疹病毒 (oHSV)瘤性病毒疗法 (OV)

更多相关视频

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Published on: May 27, 2011

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...