跨玉米同血统的转录因子结合位点差异驱动转录和表型变异
Mary Galli1, Zongliang Chen1, Tara Ghandour2
1Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, 08854-8020, USA.
bioRxiv : the preprint server for biology
|June 19, 2024
概括
研究人员绘制了玉米基因组中的转录因子 (TF) 结合部位的地图,揭示了杂交系之间的显著差异. 这种功能目录有助于了解作物特征和改善农业实践.
科学领域:
- 基因组学就是基因组学.
- 植物生物学 植物生物学
- 农业科学 农业科学
背景情况:
- 植物基因组中的调控元素对于作物发育至关重要,但仍然不太了解.
- 对转录因子 (TF) 结合部位的有限知识阻碍了作物改良策略.
研究的目的:
- 在两个关键的杂交系中映射200个玉米TF的TF绑定事件.
- 为了在整个玉米基因组中提供经验性的结合位点注释.
- 了解TF在作物特征和农业改进中具有约束力的多样性.
主要方法:
- 使用DNA亲和力净化测序 (DAP-seq) 来识别TF结合位点.
- 在B73和Mo17玉米杂交系中比较TF结合模式.
- 使用CRISPR-Cas9基因编辑来研究TF结合区域的功能影响.
主要成果:
- 绘制了 200 个玉米 TF 的 TF 结合位点,标注了基因组的 5.3%.
- 鉴定了玉米杂交系之间的TF结合的广泛差异,与结构变异和基因表达有关.
- 证明TF结合位点的变化会影响复杂的定量特征位点 (QTLs),如开花时间 (vgt1) 和草食动物耐药性 (DICE).
- 展示了编辑TF结合区域会影响基因表达和表型.
结论:
- 该研究提供了玉米TF结合事件的全面功能目录.
- 这种资源对于比较TF结合分析和了解作物遗传结构是有价值的.
- 这些发现突出了利用TF的潜在潜力,为增强作物育种和农业进步提供具有约束力的见解.
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