长寿记忆B细胞与Plasmodium falciparum merozoite抗原和变异的表面抗原之间的表型差异
Raphael A Reyes1, Louise Turner2, Isaac Ssewanyana3
1Department of Microbiology, Immunology & Molecular Genetics, Long School of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
针对Plasmodium falciparum merozoite抗原 (MSP1/AMA1) 和变异性表面抗原 (PfEMP1) 的长寿命B细胞在表型上有所不同. 这表明在疟疾流行地区有不同的免疫反应和再感染动态.
科学领域:
- 免疫学 免疫学 免疫学
- 疟疾学 疟疾学
- 寄生虫学的寄生虫学
背景情况:
- 对Plasmodium falciparum的幽默性免疫包括对美罗抗原 (例如MSP1,AMA1) 和变异性表面抗原 (例如PfEMP1) 的反应.
- 对这些抗原特异的长寿B细胞在感染后仍然存在,但它们的表型和对减少传播的反应仍然是不可比拟的.
研究的目的:
- 为了比较乌干达成年人的长期记忆和非典型B细胞的表型,特异性对 merozoite抗原 (MSP1 / AMA1) 与变异性表面抗原 (PfEMP1) 相比.
- 为了研究在P. falciparum传播减少后B细胞反应如何变化.
主要方法:
- 对乌干达成年人的抗原特异性B细胞 (向MSP1/AMA1和PfEMP1CIDRα1域) 的表型分析.
- 在P. falciparum传播减少期间 (中位数为1.7年) 之前和之后的纵向研究.
- 流式细胞计量用于表征B细胞子集 (CD95,CD11c,FcRL5,T-bet).
主要成果:
- 抗原特异性激活B细胞在减少传播后减少,但长寿B细胞仍然可检测.
- 特定于MSP1/AMA1的B细胞主要是CD95+CD11c+记忆B细胞和FcRL5-T-bet-异型B细胞.
- 特定于CIDRα1的B细胞主要是CD95-CD11c-记忆B细胞,并富含潜在参与抗原呈现的非典型B细胞子集.
结论:
- 不同的B细胞子集对不同的Plasmodium falciparum抗原做出反应,表明差异性免疫识别或处理.
- 这些表型差异可能会影响B细胞在随后的Plasmodium falciparum再感染后的反应的大小和动力学.
更多相关视频
08:46Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
Published on: May 18, 2016
10:38High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
Published on: July 17, 2014
相关概念视频
Cells of the Adaptive Immune Response
Immunological Memory
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
Special Features of Adaptive Immunity
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
