相关实验视频

Updated: Jun 23, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

使用恒星质谱仪在生物流体中开发高度复杂的向蛋白质组学试验

Deanna L Plubell¹, Philip M Remes², Christine C Wu¹

¹Department of Genome Sciences, University of Washington, Seattle WA.

bioRxiv : the preprint server for biology

|June 19, 2024

概括

我们开发了一种新的混合质谱仪,用于大规模的向蛋白质测试,使临床研究成为可能. 这种方法使用实时对齐来提高准确性和速度,克服了蛋白质组学当前的可扩展性限制.

科学领域:

蛋白质组学和质谱学
生物医学检测和临床诊断

背景情况:

针对性蛋白质测试对临床研究至关重要,但难以扩展到数千个目标.
目前的测试限制阻碍了从发现实验到大规模临床应用的过渡.

研究的目的:

通过使用一种新型混合名目质量仪表来演示大规模向蛋白质测试的生成.
克服当前针对性蛋白质组测试的可扩展性限制,用于临床应用.

主要方法:

使用了一种新的混合名目质谱仪 (StellarTM) 与实时对齐来管理移动保留时间.
使用气相分离 (GPF) 数据独立获取 (DIA) 的前体信息构建的测定.
使用轨道陷DIA和线性离子陷并行反应监测 (PRM) 与矩阵匹配的校准曲线进行比较量化.

主要成果:

成功生成了大规模的有针对性的测试,证明了对并发目标的灵敏度和速度.
验证了该方法在不同质谱仪上获得的GPF DIA图书馆调度测试的能力.
将工作流应用于脑脊髓液 (CSF) 和血中的细胞外囊泡 (EV) 蛋白质调查中的神经退行性疾病蛋白质测定.

结论:

恒星TM质谱仪可以通过实时对齐进行可扩展的向蛋白质组测试.
这种方法弥合了发现蛋白质组学和大规模临床研究之间的差距.
所示的工作流程适用于各种生物样本和疾病区域.

更多相关视频

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...