Jove
Visualize
联系我们

相关概念视频

Vaccine Production01:23

Vaccine Production

136
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
136

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Emergence of fibrotic pericytes and their transcriptional regulation in pulmonary fibrosis.

Nature communications·2026
Same author

FOXM1 inhibitor, RCM‑1, enhances venetoclax mediated apoptosis through downregulation of ATP2B4 in rhabdomyosarcoma.

International journal of oncology·2026
Same author

FOXF2 regulates pericyte-endothelial signaling required for vascular homeostasis after neonatal hyperoxic lung injury.

Nature communications·2026
Same author

Development of ACE2-tropic-betacoronavirus therapeutics for future pandemic preparedness.

Nature communications·2025
Same author

Exceptionally broad HIV-1 neutralization via bispecific antibody-mediated prepositioning.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Beyond Protocol: The Patient Behind the Checklist.

Academic medicine : journal of the Association of American Medical Colleges·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Apr 28, 2026

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
12:09

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

42.8K

设计一种针对RBD隐秘面部的SARS-CoV-2疫苗,通过免疫聚焦准RBD隐秘面部.

Theodora U J Bruun1,2, Jonathan Do1,2, Payton A-B Weidenbacher1,3

  • 1Sarafan ChEM-H, Stanford University, Stanford, CA 94305.

bioRxiv : the preprint server for biology
|June 19, 2024
PubMed
概括

使用PMD-RBD免疫原的新疫苗策略成功集中了抗体反应,以准SARS-CoV-2尖端蛋白的神秘面部. 这种方法增强了对Sarbecoviruses的广泛中和,这对未来的疫苗开发至关重要.

更多相关视频

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.3K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.6K

相关实验视频

Last Updated: Apr 28, 2026

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
12:09

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

42.8K
Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.3K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.6K

科学领域:

  • 病毒学 病毒学
  • 免疫学 免疫学 免疫学
  • 疫苗开发 疫苗开发

背景情况:

  • SARS-CoV-2 尖端蛋白的受体结合域 (RBD) 是中和抗体的关键目标.
  • 针对RBD神秘面部的抗体在Sarbecoviruses中表现出广泛的中和,但很少自然产生.

研究的目的:

  • 使用PMD (保护,修改,降低保护) 技术开发新的受体结合域 (RBD) 免疫原.
  • 将抗体反应集中在RBD的神秘面部表征上,通过像S2X259.9这样的广泛中和抗体来识别.

主要方法:

  • 使用免疫聚焦技术PMD创建专门设计的PMD-RBD免疫原体.
  • 使用PMD-RBD抗原诱导免疫反应.
  • 使用血清枯竭试验来分析多克隆抗体反应.

主要成果:

  • PMD-RBD免疫诱导了高结合度和广泛的中和活性对各种沙尔贝科病毒菌株.
  • 血清消耗分析证实,PMD成功地将抗体反应引导到RBD的神秘面部.
  • 证明了PMD克服免疫主导和重新聚焦幽默免疫的能力.

结论:

  • 这种PMD技术有效地将抗体对SARS-CoV-2 RBD的神秘表位的反应集中.
  • PMD-RBD免疫原提供了一个有希望的策略,用于开发更广泛,更有弹性的疫苗来对抗沙尔贝病毒和新出现的病毒威胁.