有证据表明RNA聚合酶III在蛋白质编码基因促进体中的招募和转录
K C Rajendra1, Ruiying Cheng2, Sihang Zhou2
1Center for Biophysics and Quantitative Biology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
bioRxiv : the preprint server for biology
|June 19, 2024
概括
RNA聚合酶III (Pol III) 在蛋白质编码基因促进体中意外发现,转录小RNAs. 这一发现影响了对基因调节和RNA生物学的理解,对癌症的结果有影响.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 在RNA生物学,RNA生物学.
背景情况:
- RNA聚合酶 (Pol I,II,III) 在RNA合成中具有不同的作用.
- 这些聚合酶之间的精确相互作用和共享功能尚未完全理解.
- 以前的研究表明偶尔会重叠,但全面的比较分析是有限的.
研究的目的:
- 综合分析和比较人类基因组中的全球占用量I,II和III.
- 研究共享或重叠的聚合酶活动的功能影响.
- 识别新的转录元件和小型非编码RNA.
主要方法:
- 对人类RNA聚合酶ChIP-seq数据集的综合分析.
- 在各种基因组元素中对聚合酶占用率的量化,包括蛋白质编码基因,非编码基因和重复元素.
- 分析新生RNA的读数和基因表达在Pol III中断时的变化.
主要成果:
- 确认了Pol I,II和III对它们已知的目标基因的正规招募.
- 发现了Pol III机器对特定蛋白质编码基因的促进者的广泛,意想不到的招募.
- 鉴定了具有小,新生的RNA读数的Pol III占用促进体,具有Pol III转录终结的特征.
- 显示Pol III中断影响了对核糖体蛋白质丰富的编码基因的表达,并与癌症的不良结果有关.
- 识别了具有对La (SSB) 破坏敏感的新兴RNA签名的未注释的基因组元素.
结论:
- RNA聚合酶III的作用比以前更广泛,包括蛋白质编码基因促进者的转录.
- 这一发现重塑了对聚合酶相互作用和基因调节的理解.
- 由Pol III转录的潜在的新型小型非编码RNA对RNA生物学和癌症研究有影响.
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