用BREX Type 4系统在人类肠道微生物群中的酸DNA修饰
Yifeng Yuan1, Michael S DeMott1,2, Shane R Byrne1
1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
bioRxiv : the preprint server for biology
|June 19, 2024
概括
微生物DNA化 (PT) 在人类肠道微生物群中很普遍,新的合成系统被发现. 这种表观遗传修饰富含rRNA基因,对氧化很敏感.
科学领域:
- 微生物学 微生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 酸化 (PT) 是一种独特的微生物DNA骨干修饰,涉及硫插入.
- 已知dnd和ssp基因家族调解PT合成.
- 了解PT的分布和功能对于微生物基因调节和宿主防御至关重要.
研究的目的:
- 调查PT基因在人类肠道微生物群中的流行和分布.
- 确定新型PT合成系统及其遗传基础.
- 描述PT修饰的基因组位置和序列背景.
主要方法:
- 对13,663个人类肠道微生物组基因组进行dnd,ssp和brx基因的全基因组分析.
- 假定新的PT系统的基因验证,包括BREX基因.
- 在226个肠道微生物群隔离物中进行质谱和PT特异性DNA测序.
主要成果:
- 分析的基因组中有6.3%含有dnd或ssp基因,主要是Bacillota,Bacteroidota和Pseudomonadota.
- 确定了几种假定新的PT系统,包括4型细菌排除 (BREX) brx基因.
- 在肠道微生物中证实了8个PT二核酸设置,PT富含rRNA基因,并在基因边界处耗尽.
结论:
- 人的肠道微生物组拥有各种各样的PT系统,包括新的系统.
- PT 修改是广泛存在的,并且在肠道微生物中表现出特定的基因组定位模式.
- 这项研究强调了微生物组作为发现 prokaryotic 表观遗传学和氧化敏感 DNA 修改的宝贵资源.
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