相关实验视频
Updated: Jun 23, 2025

13:00
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
2.3K
糖蛋白质质量控制因子malectin促进冠状病毒复制和病毒蛋白质生物发生
Jonathan P Davies1,2, Lars Plate1,2,3,4
1Department of Biological Sciences, Vanderbilt University, Nashville, TN, 37235.
bioRxiv : the preprint server for biology
|June 19, 2024
概括
马莱克丁 (MLEC) 通过与宿主蛋白质平衡网络相互作用,有助于冠状病毒的复制. 准MLEC可能为开发针对冠状病毒的广泛抗病毒疗法提供战略,包括SARS-CoV-2.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 宿主-病原体相互作用
背景情况:
- 冠状病毒 (CoV) 通过病毒非结构蛋白 (nsps) 操纵宿主蛋白质稳态 (蛋白质稳态),以促进感染.
- 了解跨nsp同源的共享宿主相互作用者至关重要,特别是随着SARS-CoV-2的出现.
研究的目的:
- 在贝塔冠状病毒中识别nsp2和nsp4的保守蛋白质稳定相互作用体.
- 研究这些相互作用体在病毒复制中的作用及其作为抗病毒点的潜力.
主要方法:
- 定量蛋白质组学用于识别宿主病毒蛋白相互作用.
- 功能性遗传查,以评估宿主因素对病毒感染的影响.
- 在感染期间分析马莱克丁 (MLEC) 与Oligosaccheryltransferase (OST) 复合物的相互作用.
主要成果:
- 确定了NSP2和NSP4的保守蛋白质稳定相互作用体,包括马莱克丁 (MLEC),它们促进了小鼠肝炎病毒感染.
- 消除MLEC显著降低了病毒标位,证明了它的亲病毒作用.
- MLEC与病毒的nsps相互作用并稳定与OST复合物的关联,增强病毒葡萄糖蛋白的产生和复制;MLEC还促进SARS-CoV-2的复制.
结论:
- 马莱克丁 (MLEC) 通过影响宿主蛋白质稳定和糖蛋白质质量控制,在调解冠状病毒感染方面发挥着重要作用.
- MLEC代表了开发泛冠状病毒抗病毒策略的潜在治疗目标.
相关概念视频
Protein Folding Quality Check in the RER
3.7K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.7K
Conjugated Proteins
18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Glycocalyx and its Functions
3.9K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
3.9K
Viral Structure
62.1K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.1K
Protein Complex Assembly
10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K

