相关实验视频

Updated: Jun 23, 2025

Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

Published on: September 20, 2011

基于AGS3的光遗传GDI诱导了GPCR独立的Gβγ信号传递和巨细胞迁移

Waruna Thotamune^1,2, Sithurandi Ubeysinghe^1,2, Chathuri Rajarathna^1,2

¹Department of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.

bioRxiv : the preprint server for biology

|June 19, 2024

概括

研究人员设计了一种基于AGS3.3的光遗传关氨酸核酸解离抑制剂 (GDI). 这种名为OptoGDI的工具可光学控制G蛋白信号,释放Gβγ亚单元以触发细胞迁移和局部信号.

科学领域:

蜂信号传输是如何进行的
分子生物学分子生物学
生物化学生物化学

背景情况:

通过G蛋白信号传递,G蛋白结合受体 (GPCR) 调解细胞反应.
关氨酸核酸解离抑制剂 (GDI),就像G蛋白信号传递3 (AGS3) 的激活剂一样,调节G蛋白活性.
对活细胞中的AGS3结构-功能和信号调节的理解有限.

研究的目的:

根据AGS3的G蛋白调节 (GPR) 动机设计一种光遗传GDI.
通过光学控制来研究GDI活动并诱导活细胞中的Gβγ信号传递.
开发一种工具来剖析GDI介导的通路并触发GPCR独立的信号.

主要方法:

在新型光遗传GDI (OptoGDI) 的结构引导工程.
使用OptoGDI来控制活细胞中的G蛋白信号传递.
评估OptoGDI释放Gβγ,产生局部PIP3,并触发巨细胞迁移的能力.

主要成果:

在光学刺激后,OptoGDI成功地招募到等离子体膜,并释放Gβγ亚单元.
针对OptoGDI的亚细胞向导致局部酸 (3,4,5) -三酸盐 (PIP3) 的产生.
OptoGDI触发了巨细胞迁移,证明了GPCR独立的Gβγ信号传递.

结论:

OptoGDI是用于光学剖析GDI介导信号通路的强大工具.
OptoGDI能够诱导GPCR独立的Gβγ信号传递在细胞和体内.
这种光遗传学方法推动了对G蛋白信号调节的研究.

关键词:

激活G蛋白信号传递3的激活剂 G 蛋白质是 G 蛋白质的组成部分.在GDI中,GDI是指GDI.在GPCR中,GPCR是指GPCR.巨细胞迁移的情况视觉遗传学视觉遗传学

更多相关视频

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Published on: April 5, 2016

相关概念视频

Activation and Inactivation of G Proteins

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...

GPCR Desensitization

GPCR Desensitization

G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...