自然发生的瘤修饰基因的功能测试为黑色素瘤发展提供了洞察力
bioRxiv : the preprint server for biology
|June 19, 2024
概括
鱼类Xiphophorus模型显示,ADGRE5基因作为一种天然的黑色素瘤抑制剂. 一个特定的ADGRE5等位基因抑制混合体中黑色素瘤的发展,为混合体不相容性和癌症病因学提供了新的见解.
科学领域:
- 进化遗传学的进化遗传学
- 癌症生物学 癌症生物学
- 发育生物学是发展生物学.
背景情况:
- 杂交不相容是物种化的一个关键因素,但其分子基础在很大程度上是未知的.
- 黑色素瘤在Xiphophorus鱼类杂交产生的遗传的瘤基因xmrk与瘤抑制剂的损失相结合.
- ADGRE5是一种G蛋白结合受体,已被确定为Xiphophorus杂交物中的瘤调节剂.
研究的目的:
- 为了研究Xiphophorus杂交物中的父母ADGRE5等位基因之间的功能差异.
- 确定ADGRE5在调节细胞增殖,迁移和黑色素瘤发展中的作用.
- 阐明导致混合不相容性和黑色素瘤病因的分子机制.
主要方法:
- 实验室细胞增殖和迁移测试使用Xiphophorus birchmanni和Xiphophorus malinche的ADGRE5等位基进行.
- 创建了表达xmrk瘤基因以及每个ADGRE5等位基因的转基因medaka模型.
- 对黑色素瘤发育和细胞行为的表型分析在体外和体外模型中进行.
主要成果:
- 与X. malinche等位基相比,X. birchmanni的ADGRE5等位基显著降低了细胞增殖和迁移.
- 在XMRK转基因医学中,X. birchmanniADGRE5等位基因的表达完全抑制了黑色素瘤的形成.
- 在X. malinche的ADGRE5等位基因在转基因medaka模型中没有显著的黑色素瘤抑制活性.
结论:
- 在Xiphophorus物种中,ADGRE5功能作为一种天然黑色素瘤抑制剂.
- ADGRE5等位基因的功能差异导致混合不兼容性和黑色素瘤易感性.
- 这项研究为黑色素瘤和混合功能障碍的遗传基础提供了新的见解.
相关概念视频
Cancer-Critical Genes II: Tumor Suppressor Genes
7.4K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.4K
Mouse Models of Cancer Study
5.5K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
5.5K
Cancer-Critical Genes I: Proto-oncogenes
8.8K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.8K
Loss of Tumor Suppressor Gene Functions
4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K
Mutagenicity and Carcinogenicity
1.2K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.2K


