重新检查的腹部体区域内神经元:GABA和谷氨酸投射神经元产生局部突触
Lucie Oriol1, Melody Chao1, Grace J Kollman1
1Department of Neurosciences, University of California, San Diego, La Jolla, United States.
bioRxiv : the preprint server for biology
|June 19, 2024
概括
腹部体区域 (VTA) GABA神经元以前被认为是局部内内神经元实际上投射到其他大脑区域. 这些投射神经元,而不是不同的内神经元,可能会调解局部VTA功能.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 神经解剖学是一个神经解剖学.
背景情况:
- 腹膜区域 (VTA) 对于奖励和动机至关重要.
- 传统上,VTA GABA神经元被认为是局部内部神经元,抑制多巴胺神经元.
- 缺少VTA内部神经元的明确标记物,与其他大脑区域不同.
研究的目的:
- 为了研究非多巴胺VTA神经元中的特定标记物 (帕尔瓦胺,索马托斯塔丁,神经素,穆阿片类受体) 是否能识别局部内部神经元.
- 为了确定这些神经元是局部突触还是向远端目标投射.
- 重新评估VTA GABA和谷氨酸神经元的功能性作用.
主要方法:
- 利用遗传标记来定义VTA神经元的种群.
- 从基因定义的VTA神经元群体到已知的VTA目标的追踪预测.
- 在VTA中检查了功能性突触连接 (抑制/刺激).
主要成果:
- 表达帕瓦胺,索马托斯塔丁,神经或片受体的VTA神经元向远程目标发射,如核,腹,侧面,以及前额叶皮层.
- 有证据表明,VTA GABA和谷氨酸投射神经元在VTA内形成功能性的局部突触.
- VTA投射神经元的局部附带可能会执行以前归因于VTA内部神经元的功能.
结论:
- 在VTA内部神经元和投射神经元之间的区别需要修订.
- 在VTA内部的局部功能可能由投射神经元的附带分支介导.
- 精细了解VTA电路对于理解奖励,动机和成机制至关重要.
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