一种微流体方法来研究酸酸酶活性对酸盐可用性的反应中的变化
Alireza Rahnama1, Manibarathi Vaithiyanathan1, Luis Briceno-Mena1
1Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, Louisiana, 70803, USA. melvina@clemson.edu.
The Analyst
|June 19, 2024
概括
微流体使藻类中性酸酶活性 (APA) 的单细胞分析成为可能,揭示了对水平的异质反应. 这凸显了研究藻类营养代谢的先进方法的需要.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 临界技术 临界技术
背景情况:
- 藻类的生长依赖,因此的可用性是一个关键因素.
- 性酸酶活性 (APA) 表明藻类状态,但缺乏单细胞分辨率.
- 目前用于APA量化的方法是耗时和试剂密集的.
研究的目的:
- 开发和验证用于藻类单细胞APA分析的微流体方法.
- 为了研究*克拉米多莫纳斯再硬化*对不同水平的异质单细胞APA反应.
- 根据藻类细胞的APA识别出不同的藻类细胞亚群.
主要方法:
- 基于陷的微流体装置被用于精确的单细胞操纵.
- 商业上可用的性酸酶 (AP) 活染料用于活动检测.
- 使用无监督机器学习 (HDBSCAN) 来分析单细胞APA数据.
主要成果:
- *Chlamydomonas reinhardtii*对不同的外源水平表现出高度异质的APA反应.
- 基础度和尖度都显著影响了APA.
- HDBSCAN确定了具有不同APA水平的不同亚种群,揭示了人口内部异质性.
结论:
- 微流体学为高分辨率单细胞APA分析提供了一个具有成本效益的平台.
- 藻类APA的特点在于单细胞水平上具有显著的异质性,具有挑战性的批量分析方法.
- 了解单细胞异质性对于准确评估藻类代谢和环境反应至关重要.
相关概念视频
Measuring Reaction Rates
Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical field in...
Brønsted-Lowry Acids and Bases
In 1923, the Brønsted–Lowry definition of acids and bases was proposed by Johannes Brønsted and Thomas Lowry. According to this theory, a Brønsted acid is defined as a species that donates a proton in a chemical reaction and gets converted to its conjugate base. A Brønsted base is defined as a species that accepts a proton in a chemical reaction and gets converted into its conjugate acid. These transfers of protons are caused by the displacement of electrons in these reactions, which is...
Acids, Bases and Neutralization Reactions
Acids and bases play several important roles in biology. The pH of a biological system can significantly impact the function of biological molecules, including enzymes, proteins, and nucleic acids. For example, enzymes have optimal pH ranges for their activity, and changes in pH can denature or alter their structure, affecting their function. Acids and bases also play a crucial role in cellular signaling and communication. The pH of the extracellular fluid around cells can influence the...
Titration of a Weak Acid with a Weak Base
Weak acids and bases do not undergo dissociation completely, and titrations between these two are rarely studied. When such studies are performed, say, for the titration of a weak acid with a weak base, the titration curve plots the change in pH as a function of the volume of base added. Take the titration of acetic acid with ammonia, for instance. During the titration, these two species form ammonium acetate and water, but the pH change is slow and gradual.
As a result, there is no simple...
As a result, there is no simple...
Titration of Polyprotic Base with a Strong Acid
The titration of a polyprotic base such as sodium carbonate with a strong acid such as hydrochloric acid results in two equivalence points on the titration curve. At the first equivalence point, the carbonate ions in the base are completely converted to bicarbonate ions. The second equivalence point corresponds to the complete conversion of bicarbonate ions to carbonic acid, which dissociates into carbon dioxide and water. The region before the first equivalence point corresponds to the...
Fast Reactions
Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...


