Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Matrix Metalloproteinase‑9 (MMP-9) Activatable Gold Nanoparticles for <i>In Situ</i> Zymography and Diagnostics of Neurofibromatosis Type 2 (NF2) Tumors.

ACS applied nano materials·2026
Same author

Single-particle surface-enhanced coherent anti-Stokes Raman scattering: Nanoparticle design and mechanism.

Science advances·2026
Same author

Enabling Technologies: Instrumentation for Specific Sensing Applications.

ACS sensors·2025
Same author

Linker-Free Synthesis of Core/Satellite Nanoparticles for Single-Particle Surface-Enhanced Raman Spectroscopy and Photocatalysis.

Nano letters·2025
Same author

Raman investigation of in vivo radiation exposure on melanin in murine hair.

PNAS nexus·2025
Same author

Reduction of Spectral Overlap in Spectral Surface-Enhanced Raman Spectroscopy Imaging Using a Dove Prism.

Applied spectroscopy·2025

相关实验视频

Updated: Jun 23, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K

数字表面增强拉曼光谱法用于在流量中量化单个分子检测.

Hannah C Schorr1, Zachary D Schultz1

  • 1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA. schultz.133@osu.edu.

The Analyst
|June 19, 2024
PubMed
概括

通过计数而不是测量强度来数字化表面增强拉曼散射 (SERS) 事件,可以显著提高分子检测. 这种新的方法实现了单分子量化,并将检测极限降低了数量级.

科学领域:

  • 分析化学 分析化学
  • 频谱学是一种光谱学.
  • 纳米技术 纳米技术

背景情况:

  • 表面增强拉曼散射 (SERS) 提供无标签的分子分析,具有潜在的单分子灵敏度.
  • 来自不同等离子体环境的信号变异性阻碍了准确的量化,特别是在低分析剂度下.
  • 传统的基于强度的检测极限 (LOD) 计算可以受到SERS信号噪声的限制.

研究的目的:

  • 开发一种用于改善SERS测量中的检测极限 (LOD) 的新方法.
  • 通过使用SERS,使单个分子在流动溶液中的可靠量化.
  • 克服SERS中传统的基于强度的LOD计算的局限性.

主要方法:

  • 通过将光谱分类为包含事件或不使用得分值来数字化SERS事件.
  • 使用多变量曲线分辨率进行光谱分析和事件分类.
  • 量化二进制"是/否"SERS事件数据以建立线性检测区域.

主要成果:

  • 数字化方法显著降低了SERS分析的检测极限 (LOD).
  • 新方法在流动溶液中实现了单分子水平的量化.
  • 与传统的基于强度的方法相比,观察到LOD的数量级改进.

更多相关视频

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.3K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.9K

相关实验视频

Last Updated: Jun 23, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.3K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.9K

结论:

  • 数字化SERS事件提供了一个强大的方法来提高灵敏度,并使单分子量化.
  • 这种方法克服了SERS固有的信号可变性问题,导致更可靠的低度分析.
  • 开发的技术将LOD推向SERS可以实现的最低物理极限.