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蛋白酶和逆转录酶二分化之间的相互作用在HIV-1多蛋白模型中

Brisa Caroline Alves Chagas¹, Xiaohong Zhou¹, Michel Guerrero¹

¹Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

Protein science : a publication of the Protein Society

|June 19, 2024

概括

逆转录酶 (RT) 二分体接口是人类免疫缺陷病毒I型 (HIV-1) Gag-Pol多蛋白二分化的关键. 破坏RT接口的突变阻止了二分化,与影响蛋白酶 (PR) 接口的突变不同.

科学领域:

病毒学病毒学
结构生物学结构生物学
生物化学生物化学

背景情况:

人类免疫缺陷病毒I型 (HIV-1) 的Gag-Pol多蛋白对病毒复制至关重要,它编码了诸如蛋白酶 (PR),逆转录酶 (RT) 和整合酶 (IN) 等关键酶.
这些酶的成熟形式存在于二聚体或四聚体,但它们的前体二聚化的机制仍然不清楚.
了解前体二分化对于开发针对HIV-1复制的新型抗病毒策略至关重要.

研究的目的:

调查前体聚蛋白中蛋白质酶 (PR) 和逆转录酶 (RT) 的二分化机制.
阐明PR和RT二元接口在PR-RT前体二元化中的作用.
评估特定突变对PR-RT二分化及其功能影响的影响.

主要方法:

在PR活性位点 (D25A) 和SUMO-tag.中具有非活化突变的模型PR-RT前体的构建和表征.
在PR (PR(T26A) -RT) 或RT (PR-RT(W401A)) 区域中制备具有二元解离突变的PR-RT突变.
尺寸排除色谱 (SEC) 用于分析单体-二元体状态并确定解离常数.
反转录酶和RT成熟测试以评估功能后果.

主要成果:

美国证券交易委员会分析显示,在RT区域发生突变的PR-RT(W401A) 突变仅作为单体存在,这表明了RT接口的关键作用.
在PR-RT和PR(T26A) -RT突变中显示出单体和二元体分数,这表明PR接口的贡献较小.
蛋白酶抑制剂达鲁纳维尔显著增强了PR-RT的缩小.
预计PR-RT二元体的解离常数与成熟的RT形式相似,这表明它在病毒组装中的相关性.

结论:

逆转录酶 (RT) 模态接口是PR-RT前体中模态化的主要决定因素.
蛋白酶 (PR) 模态接口在PR-RT前体的模态化中起到次要作用.
这些发现为HIV-1 Gag-Pol的结构组织提供了洞察力,并为抗病毒药物开发提供了潜在的目标.

关键词:

这是一种Gag-Pol多蛋白质.艾滋病毒-1病毒.达鲁纳病毒 (darunavir) 是一种病毒.分化二元化是指二元化的抑制剂抑制剂的作用蛋白质酶蛋白质酶是一种蛋白质.反向转录酶的使用

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