探索细胞悬浮物的声学度的操作边界
Amaury de Hemptinne1, Pierre Gelin2, Ilyesse Bihi2
1Department of Chemical Engineering, µFlow Group, Vrije Universiteit Brussel, 1050, Brussels, Belgium. amaury.de.hemptinne@vub.be.
Applied microbiology and biotechnology
|June 19, 2024
概括
这项研究介绍了一种微流体装置,使用声解来有效地缩细胞悬浮物. 该方法成功地将细胞与液体分离,满足高细胞度应用的工业需求.
科学领域:
- 生物技术是生物技术.
- 微流体学 微流体学
- 声学细胞聚焦 声学细胞聚焦
背景情况:
- 在连续流中缩电池悬浮物对工业应用提出了重大挑战.
- 现有的方法经常在高细胞度和多样化的多任务要求下扎.
研究的目的:
- 开发和验证一种标准化,通用的微流体装置,用于缩细胞悬浮液.
- 为了研究高度的高效细胞聚焦和分离的声解.
主要方法:
- 在微流体装置内利用声解体来聚焦细胞 (Saccharomyces cerevisiae,大肠杆菌,中国仓鼠卵巢细胞).
- 采用道设计,其中有一个用于细胞收集的中央出口和用于清除液体的侧面出口,实现了2:1的液体与细胞分离比.
- 分析了通道深度,细胞菌株特性 (尺寸,可压缩性,密度) 和流量对工艺效率的影响.
主要成果:
- 在微通道中使用声学聚焦成功集中了三种不同的细胞菌株.
- 确定增加通道深度会降低工艺效率,因为声能密度较低.
- 观察到影响聚焦行为的特定压力声学对比因素.
- 在高固体含量和流速下,已证明剪切诱导的扩散.
结论:
- 微流体学中的声学聚焦为缩高密度细胞悬浮提供了一种可行的方法.
- 了解参数相互作用 (通道深度,流速,细胞特性) 对于优化过程效率至关重要.
- 开发的设备显示了各种工业应用的潜力,需要缩的电池悬浮液.
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