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Maria Schmidt¹, Ricardo Guerreiro¹, Nadia Baig¹

¹Institute for Quantitative Genetics and Genomics of Plants, Heinrich Heine University, Düsseldorf, Germany.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|June 19, 2024

概括

玉米中大麦黄色矮人病毒 (BYDV) -PAV耐药性与两个基因有关,Zm00001eb428010和Zm00001eb428020. 在Zm00001eb428010中的特定删除通过改变蛋白质特性来赋予耐药性.

科学领域:

植物病理学植物病理学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

大麦黄矮体 (BYD) 是一种重要的谷物病毒疾病,在全球范围内造成大量的产量损失.
开发基因耐药品种对于经济和环保的比亚迪控制至关重要.

研究的目的:

确定在玉米中对BYDV-PAV耐药性的基因.
调查影响玉米易受 BYDV-PAV 的遗传变异.
分析BYDV-PAV感染对不同抵抗水平的玉米杂交品种基因表达的影响.

主要方法:

利用两个双亲映射种群来缩小对BYDV-PAV耐药性的定量特征位置 (QTL).
采用关联映射和基因表达分析来精确确定候选耐药基因.
测序候选基因以识别单核酸多态 (SNP) 和结构变异.

主要成果:

将BYDV-PAV抗性QTL降低到0.3Mbp区域,其中包含9个基因.
确定Zm00001eb428010和Zm00001eb428020作为BYDV-PAV耐药性的关键候选基因.
在耐药玉米杂交中发现了5'-UTR中的54bp删除和Zm00001eb428010中的蛋白质改变变体.

结论:

Zm00001eb428010和Zm00001eb428020在赋予BYDV-PAV耐药性方面有很大的影响.
由于Zm00001eb428010的变化而改变的蛋白质丰度或特性可能会介导抗性.
这些基因可能通过干扰病毒复制或诱导ROS信号通路来赋予耐药性.

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