产生的海洋振动体的核心转录组揭示了糖解在它们高效生产的贡献
Yoshihiro Sato1, Sayaka Mino2, Fabiano Thompson3
1Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Current microbiology
|June 19, 2024
概括
由于基因表达和成形运输机制的改变,海洋振动体从酸盐中产生低效的气. 这项研究调查了这些细菌中节能策略的潜在分子基础.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 酸盐 (Pyr) 是糖解中的关键代谢物,作为无氧海洋体中通过酸盐生成 (H) 的前体.
- 海洋活体,包括Vibrio tritonius和Vibrio porteresiae,在代谢酸盐时表现出低效的H2生产.
- 了解这种低效率背后的分子机制对于优化这些生物体中的H2生物合成至关重要.
研究的目的:
- 阐明导致海洋生物中从酸盐中产生低效的H2的分子机制.
- 调查糖解如何影响这些细菌中的H2生产.
- 为了识别 pyruvate-metabolizing 海洋vibrios 中常见的基因表达模式.
主要方法:
- 应用"核心转录组"方法来识别两个主要的H2生产维体物种中共享的基因表达.
- 对酸盐代谢细胞和葡萄糖代谢细胞之间的基因表达特征进行比较分析.
- 专注于监管网络,包括酸 (PEP) - 酸 - 氧酸盐 (PPO) 节点和PhoB,RhaR和DeoR规则.
主要成果:
- 观察到与PEP-Pyruvate-Oxalate (PPO) 节点相关的基因的调节,这表明了节能机制.
- 与葡萄糖代谢细胞相比,在酸盐代谢细胞中对氧沙酸盐/甲酸盐家族抗载体基因的上调调节.
- 确定与酸盐代谢的海洋生物中的能量缺陷相关的替代形式排除策略.
结论:
- 酸盐代谢的海洋生物中低效的H2生产与特定的基因调节和替代形式的运输有关.
- 节能策略,包括PPO节点和修改格式排除,发挥重要作用.
- 恩布登-梅耶霍夫-帕纳斯 (EMP) 路径对海洋振动体高效2生产的贡献需要进一步调查.
更多相关视频
13:51Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
Published on: February 18, 2009
12.5K
10:03Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
4.3K
相关概念视频
Energy-requiring Steps of Glycolysis
163.4K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
163.4K
Hydrolysis of ATP
75.5K
The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
75.5K
Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
Export of Mitochondrial and Chloroplast Genes
3.7K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.7K
Chemiosmosis
98.0K
Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
98.0K
Energy-releasing Steps of Glycolysis
139.0K
Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
The first energy-releasing step—the 6th step of glycolysis...
139.0K
