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Updated: Jun 23, 2025

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Published on: August 9, 2011

细菌素减弱了Listeria monocytogenes引起的肠壁功能障碍和炎症反应

Zhao Wang¹, Jing Du¹, Wenyu Ma¹

¹School of Food and Health, Beijing Technology and Business University, Beijing, 100048, China.

Applied microbiology and biotechnology

|June 19, 2024

概括

细菌素对Listeria monocytogenes具有强大的抗菌作用,改善肠道屏障功能并减少炎症. 植物素RX-8表现出最强的保护作用,为食品安全和肠道健康提供了抗生素的有希望的替代品.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
胃肠病学胃肠病学

背景情况:

细菌素是抗微生物,在治疗食源性感染和胃肠道疾病方面具有潜在的应用.
抗生素耐药性需要探索替代治疗剂.

研究的目的:

评估尼,素Gr17和植物素RX-8对Listeria monocytogenes的抗菌活性.
评估这些细菌素在缓解L. monocytogenes诱导的肠壁功能障碍和炎症方面的有效性.

主要方法:

在体外评估细菌的抗菌活性和细胞毒性.
评估细菌素对Caco-2细胞透性的影响,紧密结合蛋白组合和炎症性细胞因子分泌.
对细菌素对基激活蛋白激酶 (MAPK) 和核因子kappa B (NF-κB) 信号通路的影响的分析.

主要成果:

细菌素对L. monocytogenes表现出显著的抗菌活性,没有细胞毒性.
细菌素通过促进紧密结合蛋白 (TJP) 组装 (ZO-1,ocludin,claudin-1) 来改善Caco-2细胞屏障的完整性.
细菌素通过抑制MAPK和NF-κB通路并降低IL-6,IL-1β和TNF-α分泌来降低炎症反应.

结论:

与尼和素Gr17相比,Plantaricin RX-8在肠道细胞上表现出优异的抗菌和保护作用.
细菌素作为抗生素的有效替代品,有望对抗L. monocytogenes感染并支持肠道健康.

关键词:

具有抗菌活性,具有抗菌活性.在这种情况下,细菌素可以炎症反应炎症反应肠道上皮质屏障肠道上皮质屏障病毒性因素是病毒性因素.

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