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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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使用跨光谱矩阵适合被动化成像的反向方法.

Celestine Lachambre, Adrian Basarab, Jean-Christophe Bera

    IEEE transactions on ultrasonics, ferroelectrics, and frequency control
    |June 19, 2024
    PubMed
    概括

    我们使用一种新的交叉光谱矩阵拟合 (CMF) 方法改进了被动化成像. 这种技术提高了轴分辨率和对比度与噪声比率,用于监测高强度聚焦超声波 (HIFU) 应用.

    科学领域:

    • 医疗成像医学成像
    • 声学 声学 在声学方面
    • 超声波技术 超声波技术 超声波技术

    背景情况:

    • 高强度聚焦超声波 (HIFU) 应用,如声波和药物输送,需要监测腔位.
    • 被动声学映射提供了洞穴监测,但与脉冲回声超声相反,其轴分辨率较差.
    • 现有的方法缺乏洞化事件的精确空间定位.

    研究的目的:

    • 为了提高被动化成像中的轴分辨率.
    • 适应交叉光谱矩阵拟合 (CMF) 方法,以改进洞穴的空间映射.
    • 为了比较CMF与弹性网 (CMF-ElNet) 和稀疏总变量 (CMF-spTV) 调整的性能与现有技术.

    主要方法:

    • 开发了一种适应的交叉光谱矩阵配合 (CMF) 方法,以解决被动化成像的反向问题.
    • 应用的稀疏性促进规范化:弹性净 (ElNet) 和稀疏总变化 (spTV).
    • 使用模拟和实验数据验证了CMF方法,并将其与频率延迟和总和 (DAS) 和强大的卡光束变频器 (RCB) 进行了比较.

    主要成果:

    • 与RCB和DAS相比,CMF方法显著改善了轴分辨率,实现了全宽半最大 (FWHM) 的3倍和5倍减少.
    • 与RCB相比,基于CMF的方法在实验条件下提高了超过15dB的对比度和噪声比率 (CNR).

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  • 稀疏的总变量 (spTV) 之前显示出云形化源的优越性能.

    • 结论:

    • 适应的CMF方法为被动化成像提供了轴向分辨率的显著改善.
    • 基于CMF的技术为监测HIFU诱导的空洞化提供了卓越的对比度和空间精度.
    • 稀疏规则化,特别是spTV,对于重建复杂的化模式是有效的.