相关实验视频

Updated: Jun 23, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.2K

半任意的qPCR用于通过终端脱氧核基转移酶辅助RNA原始化DNA聚合检测循环miRNA的敏感检测

Jingyu Cui1,2, Jiafang Piao1, Houyu Han1

  • 1Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, China.

Analytical chemistry
|June 19, 2024
PubMed
概括

我们开发了一种新的方法来检测循环的microRNAs (miRNAs),使用RNA-primed DNA聚合和qPCR. 这种敏感的测试准确地量化了像miR-122这样的疾病生物标志物,即使是在非常低的水平上.

更多相关视频

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR07:37

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

8.6K
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

20.6K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.2K