植物中通过TOR激酶对光合作用活性进行翻译后调节
Stefano D'Alessandro1,2, Florent Velay1, Régine Lebrun3
1Aix Marseille Univ, CEA, CNRS, BIAM, LGBP Team, 13009 Marseille, France.
Science advances
|June 19, 2024
概括
植物生长受到一种新的信号通路的调节. 拉巴胺素 (TOR) 激酶的目标通过RSH3与 prokaryotic guanosine tetraphosphate (ppGpp) 途径连接,平衡叶绿体活性与生长.
科学领域:
- 植物生物学 植物生物学
- 分子细胞生物学 分子细胞生物学
- 生物化学 生化学
背景情况:
- 叶绿体对植物细胞至关重要,但它们的活性必须与生长保持平衡,以防止光氧化损伤.
- 拉巴胺素 (TOR) 激酶的标促进生长,而原生细胞的瓜诺辛四酸盐 (ppGpp) 途径调节叶绿体活性和光合作用.
研究的目的:
- 为了确定连接TOR激酶和ppGpp信号通路的翻译后机制.
- 阐明这种机制如何调节植物生长和叶绿体活动之间的平衡.
主要方法:
- 研究了RelA SpoT同源3 (RSH3) 和TOR复合体之间的相互作用.
- 通过TOR分析了RSH3的酸化.
- 研究了下调TOR活性对RSH3过度表达者和野生型植物的ppGpp合成和光合作用能力的影响.
主要成果:
- 确定RSH3是负责ppGpp生物合成的核编码酶,它与TOR复合体直接相互作用.
- 证明RSH3的植物特异性氨基终端区域以TOR-依赖的方式被酸化.
- 观察到下调的TOR活性增加了RSH3过度表达者的ppGpp合成,并以RSH依赖的方式降低了野生类型植物的光合作用能力.
结论:
- 建立了一个TOR-RSH3信号轴,它调节了叶绿体活动和植物生长之间的平衡.
- 这一途径代表了TOR调节器官功能的新机制,将生长信号与叶绿体反应集成在一起.
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