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病原体耐药性受到甘中NAC转录因子ScATAF1的负调节

Hengbo Wang¹, Liqian Qin², Chunyan Feng²

¹Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Agriculture, Instrumental Analysis Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China; National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya, Haikou, 572024/571101, Hainan, China.

Plant physiology and biochemistry : PPB

|June 19, 2024

概括

分析了甘和相关物种中的NAC (NAM,ATAF和CUC) 转录因子家族. 发现ScATAF1基因通过JA和SA通路负面调节植物防御反应.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
植物防御机制植物防御机制

背景情况:

NAC (NAM,ATAF和CUC) 转录因子家族是一个庞大而重要的植物基因组.
了解NAC基因功能对于作物改善和抗病能力至关重要.

研究的目的:

为了识别和描述Saccharum复合体中的NAC家族成员 (S. officinarum,S. spontaneum和Erianthus rufipilus).
研究ATAF亚家族,特别是ScATAF1基因在植物对压力和病原体的反应中的作用.

主要方法:

生物信息分析用于识别NAC基因家族成员并分析它们的进化关系.
在各种压力条件下的基因表达分析 (低温,激素治疗,病原体注射).
使用短暂的过度表达和DAB染色,对烟草中的ScATAF1进行功能分析.

主要成果:

分别确定了S. officinarum,S. spontaneum和E. rufipilus中的180,141和131个NAC成员.
ATAF子家族显示了净化选择 (Ka/Ks < 1) 和玉米的显著扩张的证据.
ScATAF1表达是由ABA,NaCl和低温诱导的,但是由SA和JA抑制的. 它的表达在易受污染的甘中更高.
烟草中ScATAF1的过度表达导致ROS的产生增加,并增加了对Ralstonia solanacearum的敏感性,这表明它在防御方面发挥了负面作用.

结论:

包括ScATAF1在内的ATAF亚家族在植物应激和防御反应中起着复杂的作用.
斯卡塔F1似乎通过JA和SA信号通路负面调节过敏反应和ROS产生.
这些发现为进一步研究甘中ATAF基因的生物功能提供了基础.

关键词:

ATAF成员的成员.在NAC转录因子的转录因子.病原体防御的病原体防御斯穆特病 (Smut Disease) 是一种疾病.甘是一种糖.

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