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需要的乳腺炎病原体在牛奶中的生物感知:从学术样本制备的新性到工业原型现场测试
Kira J Fitzpatrick1, Hayden J Rohlf1, Grant Phillips1
1XING Applied Research & Assay Development (XARAD) Division, XING Technologies Pty Ltd, QLD, 4073, Australia.
Talanta
|June 19, 2024
概括
一个新的现场便携式测试可以在70分钟内使用DNA提取和定量聚合酶链反应 (qPCR) 准确识别六种常见的牛乳炎病原体,帮助奶牛养殖者.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 分子诊断学 分子诊断学
- 生物传感器技术技术
背景情况:
- 牛乳炎是乳牛中普遍存在的传染病,由于牛奶产量和质量下降,造成了严重的经济损失.
- 当前的诊断方法往往需要在场外进行实验室分析,导致治疗和管理决策的延迟.
研究的目的:
- 为快速识别常见的牛乳腺炎病原体开发一种便携式,具有成本效益的现场测试.
- 创建一个用户友好的诊断工具,需要对奶农员工进行最低限度的培训.
主要方法:
- 开发一种基于聚合物的专有方法,从牛奶样本中快速提取病原体DNA.
- 对六种主要乳腺炎病原体进行定量聚合酶连锁反应 (qPCR) 试验的实施:金黄色葡萄球菌,Streptococcus agalactiae,Streptococcus dysgalactiae,Streptococcus uberis,Mycoplasma bovis和大肠杆菌.
- 将样品制备方法集成到实地测试的原型需求点系统中.
主要成果:
- 原型系统的样本到结果的周转时间为70分钟以下,用于对所有六种目标病原体的定量检测.
- 该系统展示了一种独立于培养的方法,用于分子识别的自动样本准备.
- 需求点系统与基于实验室的qPCR有很好的相关性,验证了其诊断准确性.
结论:
- 一种针对牛乳腺炎的新型点需要生物传感器技术已经成功开发并向商业化迈进.
- 这项技术为奶牛养殖者提供了快速,准确和可访问的诊断解决方案,可能消除了对现场实验室测试的需求.
- 该系统赋予非专家用户直接在农场对关键乳腺炎病原体的分子识别能力.
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