工程低致病性Helicobacter pylori作为口服瘤免疫调节剂,用于刺激系统性免疫反应
Zishan Zeng1, Yue Sun1, Jingwen Jiang1
1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, PR China.
Biomaterials
|June 19, 2024
概括
装有光敏感剂的工程Helicobacter pylori (H. pylori) 细菌为胃癌提供了一种新的口服治疗方法. 这种活体药物递送系统向瘤,增强免疫反应,并通过光动力学疗法通过出色的安全性消除癌细胞.
科学领域:
- 在瘤学瘤学.
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 胃癌是全球主要的健康问题.
- 杆菌 (H. pylori) 感染与胃癌患者的生存率改善有关.
- 研究H. pylori作为治疗剂和胃癌的口服药物输送系统是有必要的.
研究的目的:
- 为了设计H. pylori用于胃癌治疗.
- 开发一种近红外 (NIR) 触发系统 (Hp-Ce6),使用低致病性H. pylori与光敏感剂结合.
- 评估这种工程H. pylori系统用于胃癌治疗的疗效和安全性.
主要方法:
- 通过将光敏感剂与低致病性H. pylori结合起来,构建了工程H. pylori (Hp-Ce6).
- 评估了Hp-Ce6能够在胃酸中生存,透粘液并迁移到瘤部位的能力.
- 评估了Hp-Ce6的治疗效果,包括通过光动力学疗法破坏瘤细胞,免疫激活 (cGAS-STING通路),巨细胞重塑和整体生物安全性.
主要成果:
- 工程Hp-Ce6维持了胃酸中的活性,透了粘液,并迁移到瘤区域.
- Hp-Ce6激活了cGAS-STING通路并重塑了巨细胞的表型,作为瘤部位疫苗.
- 对Hp-Ce6的NIR辐射诱导了光动力学疗法,破坏了瘤细胞并触发了免疫细胞死亡,血液吸收和便消除最小,表明良好的生物安全性.
结论:
- 工程H. pylori (Hp-Ce6) 证明了作为胃瘤的口服输送平台的可行性.
- 这种方法利用H. pylori作为现场免疫调节剂来增强抗瘤疗效.
- Hp-Ce6为胃癌治疗提供了一个有前途的策略,它结合了向药物输送,光动力学疗法和免疫疗法.
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