通过Trichoderma asperellum T-1增强天然小麦的发酵和解构及其积极的转录反应
Qun Wang1, Jianghui Xiu1, Bingyang Liu1
1Key Laboratory of Recycling and Eco‑treatment of Waste Biomass of Zhejiang Province, School of Environmental and Natural Resources, Zhejiang University of Science and Technology, Hangzhou 310023, China.
Bioresource technology
|June 19, 2024
概括
与Trichoderma reesei QM6a相比,Trichoderma asperellum T-1在发酵天然草方面表现出卓越的细胞酶活性和更高的半纤维素和素降解率,为增强生物质利用铺平了道路.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物质的价值化 生物质的价值化
背景情况:
- 农业废物,如草,代表了大量未充分利用的资源.
- 微生物降解是释放红细胞生物质内能量潜力的关键.
- 有效的微生物菌株是有效的草发酵和生物质转化所需的.
研究的目的:
- 为了识别和表征一种用于天然草发酵的优质微生物菌株.
- 为了比较Trichoderma asperellum T-1和Trichoderma reesei QM6a的纤维素降解能力.
- 调查由T. asperellum T-1进行的吸管解构背后的分子机制.
主要方法:
- 在T. asperellum T-1和T. reesei QM6a.之间进行细胞酶生产的比较分析.
- 在小麦中量化半纤维素和红素降解率.
- 转录组分析,以了解与林氏纤维素分解有关的基因表达.
- 在固态和浸泡发酵中评估微生物性能.
主要成果:
- 在Trichoderma asperellum T-1表现出比T. reesei QM6a.高50%-102%的细胞酶活性.
- T. asperellum T-1 取得了显著的降解率:半纤维素的40%,素的42%.
- 转录组数据显示,富含碳水化合物结合模块对于草解构至关重要.
- 在固态和浸泡发酵中,T. asperellum T-1表现出强大的反应.
结论:
- 特里科德玛阿斯佩雷勒姆T-1是一种高度有效的天然草发酵菌株.
- 该菌株增强的酶活性和降解能力比T. reesei QM6a.提供了优势.
- 这些发现支持开发微生物剂,以提高生物质利用率.
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