Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

88.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.6K
Sanger Sequencing01:57

Sanger Sequencing

754.0K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.0K
RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

[Status analysis of clinical outcome for treatment of long COVID with traditional Chinese and western medicines].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2026
Same authorSame journal

Comparison of key diagnostics for probabilistic interpretation of STR mixture data generated with length-based and MPS methodologies.

Forensic science international. Genetics·2026
Same author

Dual Roles of Voltage-gated Calcium Channels and γ-Aminobutyric Acid-mediated Signaling in Modulating Neurotensin Receptor Type 2-induced Antinociception.

Anesthesiology·2026
Same author

Coronary sinus reducer implantation for refractory angina: a national audit of UK practice.

Heart (British Cardiac Society)·2026
Same author

Identification of low threshold off-target activation pathways during stimulation of carotid baroreceptor afferents in swine.

Journal of neural engineering·2026
Same author

Deoxygenative Olefin Insertion of Cyclic Alcohols Promoted by Sulfoxide Cation Radicals.

Journal of the American Chemical Society·2026

相关实验视频

Updated: Jun 23, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.1K

在NGS STR数据中的测序诱导的文物.

Yao-Yuan Liu1, Kevin Cheng1, Rebecca Just2

  • 1ESR Limited, Private Bag 92021, Auckland, New Zealand.

Forensic science international. Genetics
|June 19, 2024
PubMed
概括

下一代测序 (NGS) 中的噪音尖峰可以创建模仿真实DNA等位基因的工件. 本研究在MiSeq FGx数据中识别了这些"噪音尖峰器件",并介绍了它们与真实STR序列的检测和区分的方法.

关键词:
法医遗传学 法医遗传学在 MiSeq FGx 中使用.噪音尖峰是因为噪音尖峰.质量评分的质量分数.这是STRs.对文物进行测序.

更多相关视频

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
11:11

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

16.8K
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

9.5K

相关实验视频

Last Updated: Jun 23, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.1K
Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
11:11

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

16.8K
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

9.5K

科学领域:

  • 法医科学 法医科学 法医科学
  • 遗传学 遗传学 是一个
  • 分子生物学分子生物学

背景情况:

  • 下一代测序 (NGS) 提供了法医短并列重复 (STR) 分析的进步.
  • 在MiSeq FGx数据中的高读数噪声序列在区分真正的等位基因方面存在挑战.
  • 这些噪声序列往往以已知等位基的单基替代形式呈现.

研究的目的:

  • 调查在MiSeq FGx DNA 档案中观察到的高读数噪声序列的起源和特征.
  • 为了识别和描述特定的文物,称为"噪音尖峰文物",在测序过程中产生.
  • 开发检测和区分这些文物与真实的STR等位基因的方法.

主要方法:

  • 使用MiSeq FGx测序系统和ForenSeq DNA签名准备套件生成的DNA配置文件的分析.
  • 检查特征基替代和它们的位置的噪声序列.
  • 对噪声事件进行映射,以在序列运行中识别"噪声峰值".
  • 在测序-通过-合成 (SBS) 循环中研究噪音尖峰的起源.

主要成果:

  • 高读数噪声序列往往来自于在片内的特定"噪声尖峰"位置的基位替换.
  • 这些噪音尖峰发生在跑道内的一致位置,但在不同跑道的位置和振幅上有所不同.
  • 大多数有问题的噪音序列被归类为来自这些特定位置的"噪音尖峰工件".

结论:

  • 在MiSeq FGx STR分析中,噪音尖峰器件是一个重大挑战.
  • 了解SBS周期期间这些文物的起源对于准确的解释至关重要.
  • 使用MiSeq FGx数据的法医实验室对于检测和区分噪声尖峰器件的方法至关重要.