通过Cupriavidus necator通过固态发酵使用补充玉米加工副产品的聚3-基酸盐生产:在瓶子和生物反应器尺度上进行培养
Mohammad Sadegh Jafari1, Parisa Hejazi1
1Biotechnology Research Laboratory, School of Chemical, Petroleum and Gas Engineering, Iran University of Science and Technology, Tehran 16846-13114, Iran.
Journal of biotechnology
|June 19, 2024
概括
这项研究探讨了使用粮食废弃物,如玉米胚芽和来生产聚3-基酸盐 (PHB). 使用奶酪乳清和糖蜜进行优化发酵显著提高了PHB产量,使生物塑料更具成本效益.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 可持续化学 可持续化学
背景情况:
- 聚3-基酸盐 (PHB) 是一种可生物降解的聚合物,具有作为传统塑料可持续替代品的潜力.
- 目前,高昂的生产成本限制了PHB的广泛采用.
- 利用低成本,丰富的原材料对于提高PHB生产的经济可行性至关重要.
研究的目的:
- 调查使用食品加工副产品作为PHB生产的低成本基质.
- 为了优化固态发酵条件以提高PHB产量.
- 为了评估PHB生产效率,无论是在瓶和生物反应器尺度上.
主要方法:
- 固态发酵使用玉米芽和玉米作为基质.
- 补充糖蜜和奶酪乳糖以提高营养的可用性.
- 优化发酵参数,包括基质组成,温度,水分和营养添加,使用一次因素技术.
- 在包装式生物反应器中进行扩大规模的研究,以评估通风的影响.
主要成果:
- 尺度PHB生产的最佳基质成分是玉米芽与玉米的质量比为1:1,补充20% (v/w) 奶酪乳清,在72小时后达到4.1g/kg初始干重基质 (IDWS) 的产量.
- 生物反应器规模的实验表明PHB产量显著增加,在72小时后达到8.4g/kg的IDWS,代表了100%以上的改善.
- 生物反应器的最佳通风率被确定为0.5L/kg IDWS·h).
结论:
- 食品加工副产品可以通过固态发酵有效地被用作PHB生产的低成本基质.
- 优化基质成分和发酵条件,包括通风,显著提高PHB产量.
- 这些发现表明,降低PHB生产成本的可行途径,促进其作为可持续生物塑料的更广泛应用.
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