在Bacillus megaterium中微封装Vip3Ag4蛋白的紫外线保护和杀虫活性
Leopoldo Palma1, Iñigo Ruiz de Escudero2, Francisco Mañeru-Oria2
1Instituto de Biotecnología y Biomedicina (BIOTECMED), Departamento de Genética, Universitat de València, 46100, Burjassot, Spain.
概括
巨杆菌封装保护Vip3Ag4杀虫蛋白免受紫外线损伤. 这种细菌封装提供了适度的紫外线保护,增强了蛋白质对潜在杀虫剂配方的稳定性.
科学领域:
- 农业昆虫学 农业昆虫学
- 微生物生物技术 微生物生物技术
- 生物化学 生化学
背景情况:
- 可分泌的Vip3Ag4蛋白质具有杀虫性能,但易受紫外线无活化影响.
- 开发稳定的杀虫剂配方需要保护活性成分免受环境退化.
研究的目的:
- 在封装Vip3Ag4蛋白时评估Bacillus megaterium的紫外线光保护能力.
- 评估封装对暴露于紫外线的Vip3Ag4杀虫活性的影响.
主要方法:
- 在Bacillus megaterium中封装可分泌的Vip3Ag4蛋白质.
- 定量生物测试以确定紫外线无活化值.
- 封装和非封装Vip3Ag4蛋白暴露于紫外线.
主要成果:
- 非封装的Vip3Ag4蛋白在紫外线暴露后,对Spodoptera littoralis幼虫的LC50为518 ng/cm2.
- 封装的Vip3Ag4蛋白在紫外线暴露后,对Spodoptera littoralis幼虫的LC50为479 ng/cm2.
- 巨杆菌对封装的Vip3Ag4蛋白质进行了适度的紫外线保护.
结论:
- 巨杆菌封装为Vip3Ag4蛋白提供了适度的紫外线光保护.
- 封装的Vip3Ag4蛋白在紫外线暴露后保持显著的杀虫活性.
- B. megaterium 作为开发具有增强稳定性的新型杀虫剂配方的潜在平台.
关键词:
虫巨杆菌 (Bacillus megaterium) 是一种有着巨杆菌的动物.细菌 thuringiensis 这种疾病.生物杀虫剂配方 生物杀虫剂配方生物控制是生物控制.昆虫虫害虫害虫害虫害虫害虫害害虫害虫害虫害虫害虫害害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害微封装是一种微封装.普里斯蒂亚巨动物群 (Priestia megaterium) 是一个巨动物群.在Vip3蛋白质中.更多相关视频
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