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Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
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高容量临床SARS-CoV-2分子测试使用组合组合的聚合.

Shosh Zismanov1,2, Bar Shalem3, Yulia Margolin-Miller4

  • 1Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

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一个新的单阶段聚合测试策略,P-BEST,在高SARS-CoV-2阳性率期间显著减少了PCR测试. 这种方法为病原体监测和流行病应对提供了一种具有成本效益的方法.

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科学领域:

  • 传染病流行病学 传染病流行病学
  • 分子诊断学 分子诊断学
  • 公共卫生监督 公共卫生监督

背景情况:

  • 随着COVID-19大流行,对SARS-CoV-2检测产生了巨大的需求,导致全球延迟.
  • 由于实际限制,传统的聚合测试方法仅限于较低的阳性率 (<2%).

研究的目的:

  • 为有效检测SARS-CoV-2开发和验证一个单阶段的聚合测试策略.
  • 评估这种策略在高病毒患病率时期的临床效用.

主要方法:

  • 开发和临床验证P-BEST (通过增强单阶段测试进行集体测试).
  • 使用3636个样本进行并排临床验证.
  • 在以色列实施在阿尔法和三角洲波期间进行大规模测试.

主要成果:

  • 在检测阳性样本和估计Ct值方面,P-BEST表现出高准确度.
  • 2021年临床应用测试了超过837,000个样本,PCR测试减少了3.1倍.
  • 即使积极性率超过10%时,该策略仍然有效.

结论:

  • P-BEST 作为大型单阶段聚合测试的概念验证.
  • 它为病原体监测和流行病应对提供了降低成本和提高效率.
  • 一个基于平板电脑的解决方案可以在资源有限的环境中方便手动组合.