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相关概念视频

Insertion of Multi-pass Transmembrane Proteins in the RER01:29

Insertion of Multi-pass Transmembrane Proteins in the RER

The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...
Polymer Classification: Stereospecificity01:26

Polymer Classification: Stereospecificity

Polymerization generates chiral centers along the entire backbone of a polymer chain. Accordingly, the stereochemistry of the substituent group has a significant effect on polymer properties. Polymers formed from monosubstituted alkene monomers feature chiral carbons at every alternate position in the polymer backbone. Relative to the predominant orientation of substituents at the adjacent chiral carbons, the polymer can exist in three different configurations: isotactic, syndiotactic, and...

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相关实验视频

Updated: Jun 13, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

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多尺度拓学将细胞分类为亚细胞空间转录组

Katherine Benjamin1, Aneesha Bhandari2,3, Jessica D Kepple2,3

  • 1Mathematical Institute, University of Oxford, Oxford, UK.

Nature
|June 19, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了使用空间转录组学数据进行细胞类型识别的新多尺度方法. 这种方法增强了细胞分类和组织空间关系分析,而不依赖成像.

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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相关实验视频

Last Updated: Jun 13, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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科学领域:

  • 基因组学
  • 生物信息学
  • 计算生物学

背景情况:

  • 空间转录组提供现场基因表达,但在分辨率,深度和样本大小方面面临权衡.
  • 目前集成图像细分的方法受到成像质量和组织异质性的限制.
  • 基于数组的技术提供细胞下分辨率的全转录组数据,但缺乏细胞注释工具.

研究的目的:

  • 使用空间转录学开发一种新的多尺度计算框架,用于自动化的细胞类型分类.
  • 利用转录组和空间背景进行亚细胞水平的细胞识别.
  • 创建适用于多种空间转录论平台的可通用管道.

主要方法:

  • 一种多尺度的方法,结合了细胞分类的转录基因数据和空间背景.
  • 针对目标和全转录组空间平台的应用.
  • 与多参数持久同质性的集成用于细胞空间关系的拓分析.

主要成果:

  • 在人类组织中改善细胞分类和形态分析.
  • 在没有图像数据的情况下精确定位稀疏分布的小鼠免疫细胞.
  • 在狼性炎的小鼠模型中确定细胞空间关系,经过实验验证.

结论:

  • 拟议的框架允许从空间转录组数据中自动识别高分辨率的细胞类型.
  • 它克服了基于图像的方法的局限性,并在不同的技术中进行了概括.
  • 这条管道将基因表达和组织层面的生物学发现联系起来.